Publikation:

KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

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Betz_KlenTaq.pdf
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Datum

2012

Autor:innen

Malyshev, Denis A.
Lavergne, Thomas
Dwyer, Tammy J.
Ordoukhanian, Phillip
Romesberg, Floyd E.

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http://nbn-resolving.de/urn:nbn:de:bsz:352-196996
DOI (zitierfähiger Link)
https://doi.org/10.1038/nchembio.966
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Chemie: Publikationen
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Published

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Nature Chemical Biology. 2012, 8(7), pp. 612-614. ISSN 1552-4450. eISSN 1552-4469. Available under: doi: 10.1038/nchembio.966

Zusammenfassung

Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair,dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

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570 Biowissenschaften, Biologie

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ISO 690BETZ, Karin, Denis A. MALYSHEV, Thomas LAVERGNE, Wolfram WELTE, Kay DIEDERICHS, Tammy J. DWYER, Phillip ORDOUKHANIAN, Floyd E. ROMESBERG, Andreas MARX, 2012. KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry. In: Nature Chemical Biology. 2012, 8(7), pp. 612-614. ISSN 1552-4450. eISSN 1552-4469. Available under: doi: 10.1038/nchembio.966
BibTex
@article{Betz2012-07KlenT-19699,
  year={2012},
  doi={10.1038/nchembio.966},
  title={KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry},
  number={7},
  volume={8},
  issn={1552-4450},
  journal={Nature Chemical Biology},
  pages={612--614},
  author={Betz, Karin and Malyshev, Denis A. and Lavergne, Thomas and Welte, Wolfram and Diederichs, Kay and Dwyer, Tammy J. and Ordoukhanian, Phillip and Romesberg, Floyd E. and Marx, Andreas}
}
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