Publikation: Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation
Lade...
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2001
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
ChemPhysChem. 2001, 2(6), pp. 392-396. ISSN 1439-4235. eISSN 1439-7641. Available under: doi: 10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7
Zusammenfassung
In vivo microscopy of the Green Fluorescent Protein (GFP), the most important label in cell biology, with single‐molecule sensitivity is hampered by an insufficient signal‐to‐noise ratio. A significant improvement is obtained with a novel two‐color excitation technique. The picture clearly shows the increased brightness of GFP in in vitro single‐molecule assays and in live‐cell microscopy under two‐color illumination (upper cell) as compared to normal illumination (lower cell).
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
JUNG, Gregor, Jens WIEHLER, Boris STEIPE, Christoph BRÄUCHLE, Andreas ZUMBUSCH, 2001. Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation. In: ChemPhysChem. 2001, 2(6), pp. 392-396. ISSN 1439-4235. eISSN 1439-7641. Available under: doi: 10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7BibTex
@article{Jung2001-06-18Singl-42316, year={2001}, doi={10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7}, title={Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation}, number={6}, volume={2}, issn={1439-4235}, journal={ChemPhysChem}, pages={392--396}, author={Jung, Gregor and Wiehler, Jens and Steipe, Boris and Bräuchle, Christoph and Zumbusch, Andreas} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/42316"> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Wiehler, Jens</dc:creator> <dc:contributor>Jung, Gregor</dc:contributor> <dc:creator>Bräuchle, Christoph</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42316"/> <dcterms:issued>2001-06-18</dcterms:issued> <dc:creator>Steipe, Boris</dc:creator> <dc:creator>Jung, Gregor</dc:creator> <dc:contributor>Steipe, Boris</dc:contributor> <dcterms:title>Single‐Molecule Microscopy of the Green Fluorescent Protein Using Simultaneous Two‐Color Excitation</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-05-11T13:13:42Z</dc:date> <dcterms:abstract xml:lang="eng">In vivo microscopy of the Green Fluorescent Protein (GFP), the most important label in cell biology, with single‐molecule sensitivity is hampered by an insufficient signal‐to‐noise ratio. A significant improvement is obtained with a novel two‐color excitation technique. The picture clearly shows the increased brightness of GFP in in vitro single‐molecule assays and in live‐cell microscopy under two‐color illumination (upper cell) as compared to normal illumination (lower cell).</dcterms:abstract> <dc:contributor>Wiehler, Jens</dc:contributor> <dc:contributor>Bräuchle, Christoph</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-05-11T13:13:42Z</dcterms:available> <dc:language>eng</dc:language> <dc:creator>Zumbusch, Andreas</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dc:contributor>Zumbusch, Andreas</dc:contributor> </rdf:Description> </rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein