Publikation:

A semi-in-vivo preparation for optical recording of the insect brain

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Galizia97_JNeurosciMeth.pdf
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1997

Autor:innen

Joerges, Jasdan
Küttner, Armin
Faber, Till
Menzel, Randolf

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Published

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Journal of Neuroscience Methods. 1997, 76(1), pp. 61-69. ISSN 0165-0270. Available under: doi: 10.1016/S0165-0270(97)00080-0

Zusammenfassung

We describe a method for optically recording neuronal activity from an intact insect brain, upon natural sensory stimulation. In this preparation, the head capsule of the honeybee, Apis mellifera, is isolated from the body while leaving the entire brain undamaged. In short, a hole is first cut into the cuticule to allow optical access to the brain and to allow the removal of tracheae and glands. Then the head is cut free and placed into a dye-loaded and cooled ringer solution in a staining chamber for 1 h. Subsequently, the head is fixed in a recording chamber, covered with a cover-slip, and imaged under the microscope with a cooled CCD camera. The whole preparation leaves the antennae dry, free to move, and functional throughout the experiment, allowing for natural odour stimulation of the olfactory system. Using calcium sensitive or potential sensitive dyes (calcium-green or RH795), we could record the processing of olfactory information at the glomerular level in the antennal lobe of the bee.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Optical Images, Calcium green, RH 795, Olfaction, Antennal lobe, Apis mellifera

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ISO 690GALIZIA, C. Giovanni, Jasdan JOERGES, Armin KÜTTNER, Till FABER, Randolf MENZEL, 1997. A semi-in-vivo preparation for optical recording of the insect brain. In: Journal of Neuroscience Methods. 1997, 76(1), pp. 61-69. ISSN 0165-0270. Available under: doi: 10.1016/S0165-0270(97)00080-0
BibTex
@article{Galizia1997semii-7654,
  year={1997},
  doi={10.1016/S0165-0270(97)00080-0},
  title={A semi-in-vivo preparation for optical recording of the insect brain},
  number={1},
  volume={76},
  issn={0165-0270},
  journal={Journal of Neuroscience Methods},
  pages={61--69},
  author={Galizia, C. Giovanni and Joerges, Jasdan and Küttner, Armin and Faber, Till and Menzel, Randolf}
}
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