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Oxidation of glycerol, lactate, and propionate by Propionibacterium freudenreichii in a poised-potential amperometric culture system

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1990

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Emde, Rainer

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Archives of Microbiology. 1990, 153(5), pp. 506-512. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248435

Zusammenfassung

Growth of Propionibacterium freudenreichii was studied with glycerol, lactate, and propionate as energy sources and a three-electrode poised-potential amperometric electrode system with hexacyanoferrate (III) as mediator. In batch culture experiments with glycerol and lactate as substrates, hexacyanoferrate (III) was completely reduced. Growth yields increased and the fermentation patterns were shifted towards higher acetate formation with increasing hexacyanoferrate (III) concentrations (0.25- 8.0 raM). In experiments with regulated electrodes, glycerol, lactate, and propionate were oxidized to acetate and CO2, and the electrons were quantitatively transferred to the working electrode. Growth yields of 29.0, 13.4 and 14.2 g cell material per mol were calculated, respectively. The high cell yield obtained during propionate oxidation cannot be explained solely by substrate level phosphorylation indicating that additional energy was conserved via electron transport hosphorylation. Furthermore, this result indicated complete reversibility of the methyl-malonyl-CoA pathway in propionic acid bacteria.

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570 Biowissenschaften, Biologie

Schlagwörter

Propionic acid bacteria, Propionibacterium sp., Methyl-malonyl-CoA pathway, Electron transport phosphorylation, Hexacyanoferrate (III)

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ISO 690EMDE, Rainer, Bernhard SCHINK, 1990. Oxidation of glycerol, lactate, and propionate by Propionibacterium freudenreichii in a poised-potential amperometric culture system. In: Archives of Microbiology. 1990, 153(5), pp. 506-512. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248435
BibTex
@article{Emde1990Oxida-7500,
  year={1990},
  doi={10.1007/BF00248435},
  title={Oxidation of glycerol, lactate, and propionate by Propionibacterium freudenreichii in a poised-potential amperometric culture system},
  number={5},
  volume={153},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={506--512},
  author={Emde, Rainer and Schink, Bernhard}
}
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