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Cryofixation : A Tool In Biological Ultrastructural Research

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1982

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Bachmann, Luis

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https://doi.org/10.1016/S0074-7696(08)61676-9
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International Review of Cytology. Elsevier. 1982, 79, pp. 237-304. ISSN 0074-7696. Available under: doi: 10.1016/S0074-7696(08)61676-9

Zusammenfassung

This chapter describes the technique for cryofixation. The goal of ultrastructural fixation methods is to preserve the momentary distribution of all components in a system. For molecular systems, this implies the maintenance of the statistical distribution of all solute components in the solvent and the preservation of the size and shape of the macromolecules in the hydrated state. Cryofixation and freeze-storage of biological materials are important problems in many regards: for the storage of living materials, for the storage and processing of proteins, and for the preservation of structural details. The main problem of cryofixation is that the “fixed” structures are thermodynamically unstable at low temperatures. It is important to have several different cryofixation techniques at hand, if the variety of biological materials and the broad spectrum of secondary techniques are considered, whereby each has its requirements.

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570 Biowissenschaften, Biologie

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ISO 690PLATTNER, Helmut, Luis BACHMANN, 1982. Cryofixation : A Tool In Biological Ultrastructural Research. In: International Review of Cytology. Elsevier. 1982, 79, pp. 237-304. ISSN 0074-7696. Available under: doi: 10.1016/S0074-7696(08)61676-9
BibTex
@article{Plattner1982Cryof-55077,
  year={1982},
  doi={10.1016/S0074-7696(08)61676-9},
  title={Cryofixation : A Tool In Biological Ultrastructural Research},
  volume={79},
  issn={0074-7696},
  journal={International Review of Cytology},
  pages={237--304},
  author={Plattner, Helmut and Bachmann, Luis}
}
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