Immunolocalization of pathogen effectors

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2011
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Methods in Molecular Biology
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Zusammenfassung

The use of polyclonal antibodies enables the detection of proteins on a cellular and even subcellular level. Immunolocalization can be used on all pathosystems even if one or both partners of the interaction are unamenable to molecular tools like transformation. This chapter provides detailed information about how to obtain high quality antibodies, how to prepare samples, and finally how to detect the proteins. Methods for light and electron microscopy are presented.

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570 Biowissenschaften, Biologie
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Immunolocalization, polyclonal antibodies, microscopy, effector
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ISO 690KEMEN, Eric, Kurt MENDGEN, Ralf T. VOEGELE, 2011. Immunolocalization of pathogen effectors. In: Methods in molecular biology (Clifton, N.J.). 2011, 712, pp. 211-225. ISSN 1064-3745. eISSN 1940-6029. Available under: doi: 10.1007/978-1-61737-998-7_17
BibTex
@article{Kemen2011Immun-19658,
  year={2011},
  doi={10.1007/978-1-61737-998-7_17},
  title={Immunolocalization of pathogen effectors},
  volume={712},
  issn={1064-3745},
  journal={Methods in molecular biology (Clifton, N.J.)},
  pages={211--225},
  author={Kemen, Eric and Mendgen, Kurt and Voegele, Ralf T.}
}
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