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Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei

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Kinetics_of_pyrophosphate.pdf
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Datum

2005

Autor:innen

Moraes Moreira, Bernardo Luis
Soares Medeiros, Lia Carolina A.
Miranda, Kildare
Souza, Wanderley de
Barrabin, Hector

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-42910
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.bbrc.2005.06.205
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Open Access-Veröffentlichung
Open Access Green

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Biologie: Publikationen
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Published

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Biochemical and Biophysical Research Communications. 2005, 334(4), pp. 1206-1213. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2005.06.205

Zusammenfassung

In this work, we show the kinetics of pyrophosphate-driven H+ uptake by acidocalcisomes in digitonin-permeabilized promastigotes of Leptomonas wallacei. The vacuolar proton pyrophosphatase activity was optimal in the pH range of 7.5 8.0, was inhibited by imidiodiphosphate, and was completely dependent on K+ and PPi. H+ was released with the addition of Ca2+, suggesting the presence of a Ca2+/H+ antiport. In addition, X-ray elemental mapping associated with energy-filtering transmission electron microscopy showed that most of the Ca, Na, Mg, P, K, Fe, and Zn were located in acidocalcisomes. L. wallacei immunolabeled with antibodies against Trypanosoma cruzi pyrophosphatase show intense fluorescence in cytoplasmatic organelles of size and distribution similar to the acidocalcisomes. Altogether, the results show that L. wallacei acidocalcisomes possess a H+-pyrophosphatase with characteristics of type I V-H+-PPase. However, we did not find any evidence, either for the presence of H+-ATPases or for Na+/H+ exchangers in these acidocalcisomes.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Pyrophosphatase, Acidocalcisome, Tripanosomatid, Leptomonas, Proton transport, Iron, Zinc, Calcium, Vacuole, H+-PPase

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ISO 690MORAES MOREIRA, Bernardo Luis, Lia Carolina A. SOARES MEDEIROS, Kildare MIRANDA, Wanderley de SOUZA, Joachim HENTSCHEL, Helmut PLATTNER, Hector BARRABIN, 2005. Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei. In: Biochemical and Biophysical Research Communications. 2005, 334(4), pp. 1206-1213. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2005.06.205
BibTex
@article{MoraesMoreira2005Kinet-6950,
  year={2005},
  doi={10.1016/j.bbrc.2005.06.205},
  title={Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei},
  number={4},
  volume={334},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={1206--1213},
  author={Moraes Moreira, Bernardo Luis and Soares Medeiros, Lia Carolina A. and Miranda, Kildare and Souza, Wanderley de and Hentschel, Joachim and Plattner, Helmut and Barrabin, Hector}
}
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