Visualizing Cholesterol Uptake by Self-Assembling Rhodamine B-Labeled Polymer Inside Living Cells via FLIM-FRET Microscopy

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Macromolecular Bioscience. Wiley. 2020, 20(1), 1900081. ISSN 1616-5187. eISSN 1616-5195. Available under: doi: 10.1002/mabi.201900081
Zusammenfassung

Atherosclerosis is a widespread and hazardous disease characterized by the formation of arterial plaques mostly composed of fat, cholesterol, and calcium ions. The direct solubilization of cholesterol represents a promising, atheroprotective strategy to subside lipid blood levels and reverse atherosclerosis. This study deals with the in-depth analysis of polymer-mediated cholesterol dissolution inside living human cells. To this end, a recently described multifunctional block-polymer is labeled with Rhodamine B (RhoB) to investigate its interaction with cells via fluorescence microscopy. This gives insight into the cellular internalization process of the polymer, which appears to be clathrin- and caveolae/raft-dependent endocytosis. In cell single particle tracking reveals an active transport of RhoB polymer including structures. Förster resonance energy transfer (FRET) measurements of cells treated with a fluorophore-tagged cholesterol derivative and the RhoB polymer indicates the uptake of cholesterol by the polymeric particles. Hence, these results present a first step toward possible applications of cholesterol-absorbing polymers for treating atherosclerosis.

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540 Chemie
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biological application of polymers, fluoropolymers, imaging, nanoparticles, self‐assembly
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ISO 690DOLL, Franziska, Philipp KECKEIS, Patricia SCHEEL, Helmut CÖLFEN, 2020. Visualizing Cholesterol Uptake by Self-Assembling Rhodamine B-Labeled Polymer Inside Living Cells via FLIM-FRET Microscopy. In: Macromolecular Bioscience. Wiley. 2020, 20(1), 1900081. ISSN 1616-5187. eISSN 1616-5195. Available under: doi: 10.1002/mabi.201900081
BibTex
@article{Doll2020-01Visua-46167,
  year={2020},
  doi={10.1002/mabi.201900081},
  title={Visualizing Cholesterol Uptake by Self-Assembling Rhodamine B-Labeled Polymer Inside Living Cells via FLIM-FRET Microscopy},
  number={1},
  volume={20},
  issn={1616-5187},
  journal={Macromolecular Bioscience},
  author={Doll, Franziska and Keckeis, Philipp and Scheel, Patricia and Cölfen, Helmut},
  note={Article Number: 1900081}
}
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