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A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognize human cells encoding hepatitis C virus (HCV) proteins

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2004_Engler-Vaccine_04.pdf
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2004

Autor:innen

Engler, Olivier
Schwendener, Reto
Dai, Wen Juan
Wölk, Benno
Pichler, Werner
Moradpour, Darius
Cerny, Andreas

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Vaccine. 2004, 23(1), pp. 58-68. ISSN 0264-410X. Available under: doi: 10.1016/j.vaccine.2004.05.009

Zusammenfassung

Virus specific T cell responses play an important role in resolving acute hepatitis C virus (HCV) infections. Using the HLA-A2.1 transgenic mouse model we investigated the potential of a liposomal peptide vaccine to prime a CD8+ T cell response against 10 different HCV epitopes, relevant for human applications. We were able to demonstrate the induction of strong cytotoxic T cell responses and high numbers of IFN- - secreting cells, which persisted at high levels for at least 3 months. Co-integrating CpG oligonucleotides into liposomes further increased the number of IFN- -secreting cells by 2–10-fold for most epitopes tested. The frequency of specific cells was further analysed with chimeric A2 tetramers bearing the NS31073-1081 epitope and was estimated at 2–23% of the CD8+ T cell population. Importantly, mouse effector cells, specific for this epitope, were also capable of lysing a human target cell line expressing HCV proteins. This finding and the specific protection observed in challenge experiments with recombinant vaccinia virus expressing HCV sequences emphasise the biological relevance of the vaccine-induced immune response. In conclusion, such liposome formulations represent a safe and promising strategy to stimulate the CD8+ T cell against HCV.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Hepatitis C virus, Liposomes, Peptide immunisation

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ISO 690ENGLER, Olivier, Reto SCHWENDENER, Wen Juan DAI, Benno WÖLK, Werner PICHLER, Darius MORADPOUR, Thomas BRUNNER, Andreas CERNY, 2004. A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognize human cells encoding hepatitis C virus (HCV) proteins. In: Vaccine. 2004, 23(1), pp. 58-68. ISSN 0264-410X. Available under: doi: 10.1016/j.vaccine.2004.05.009
BibTex
@article{Engler2004-11-15lipos-14287,
  year={2004},
  doi={10.1016/j.vaccine.2004.05.009},
  title={A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognize human cells encoding hepatitis C virus (HCV) proteins},
  number={1},
  volume={23},
  issn={0264-410X},
  journal={Vaccine},
  pages={58--68},
  author={Engler, Olivier and Schwendener, Reto and Dai, Wen Juan and Wölk, Benno and Pichler, Werner and Moradpour, Darius and Brunner, Thomas and Cerny, Andreas}
}
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