Online saw-bioaffinity-mass spectrometry : new tool for simultaneous detection, structure determination and affinity quantification of protein-ligand interactions from biological material

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Journal of Peptide Science. Wiley. 2014, 20(S1), pp. S301-S302. ISSN 1075-2617. eISSN 1099-1387. Available under: doi: 10.1002/psc.2688
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Bioaffinity analysis using biosensors such as surface plasmon resonance has become an established technique for the detection and quantification of biomolecular interactions. However, a principal limitation of biosensors is their lack of providing structure analysis of affinity-bound ligands. We have developed an online combination of a surface acoustic wave (SAW) biosensor with electrospray ionization mass spectrometry (SAW-ESI-MS) that enables the simultaneous structure determination and affinity quantification of biopolymer ligands, dissociated from the biopolymer- ligand complex on a gold chip. Key tool of the SAW-MS combination is an interface that provides sample concentration and in-situ desalting for the MS analysis of the ligand eluate. Recent applications of the online bioaffinity- MS show broad bioanalytical potential for direct interaction studies from biological material, as diverse as antigen-antibody and lectin- carbohydrate complexes; affinity binding constants (KD) are determined from milli- to nanomolar ranges [1,2]. Moreover, we report here first applications of the online- SAW- MS to the direct top-down structural characterization of proteolytic intermediates and oligomers in the aggregation of Parkinson’s Disease key protein, alpha-synuclein (αSyn) [3] from brain homogenate.

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ISO 690SLAMNOIU, Stefan, Mihaela STUMBAUM, Camelia VLAD, Kathrin LINDNER, Christiaan KARREMAN, Marcel LEIST, Michael PRZYBYLSKI, 2014. Online saw-bioaffinity-mass spectrometry : new tool for simultaneous detection, structure determination and affinity quantification of protein-ligand interactions from biological material. In: Journal of Peptide Science. Wiley. 2014, 20(S1), pp. S301-S302. ISSN 1075-2617. eISSN 1099-1387. Available under: doi: 10.1002/psc.2688
BibTex
@article{Slamnoiu2014Onlin-52649,
  year={2014},
  doi={10.1002/psc.2688},
  title={Online saw-bioaffinity-mass spectrometry : new tool for simultaneous detection, structure determination and affinity quantification of protein-ligand interactions from biological material},
  number={S1},
  volume={20},
  issn={1075-2617},
  journal={Journal of Peptide Science},
  pages={S301--S302},
  author={Slamnoiu, Stefan and Stumbaum, Mihaela and Vlad, Camelia and Lindner, Kathrin and Karreman, Christiaan and Leist, Marcel and Przybylski, Michael},
  note={Meeting Abstract}
}
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