Publikation:

Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter

Lade...
Vorschaubild

Dateien

Schultz_2-ljyj5ohgc0sf4.pdf
Schultz_2-ljyj5ohgc0sf4.pdfGröße: 1.29 MBDownloads: 85

Datum

2022

Autor:innen

Schultz, Júnia
Modolon, Flúvio
Rosado, Alexandre S.
Sweet, Michael
Peixoto, Raquel S.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Link zur Lizenz

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Gold
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

mSystems. American Society for Microbiology. 2022, 7(4), 00367-22. ISSN 2379-5077. eISSN 2379-5077. Available under: doi: 10.1128/msystems.00367-22

Zusammenfassung

The vast majority of environmental microbes have not yet been cultured, and most of the knowledge on coral-associated microbes (CAMs) has been generated from amplicon sequencing and metagenomes. However, exploring cultured CAMs is key for a detailed and comprehensive characterization of the roles of these microbes in shaping coral health and, ultimately, for their biotechnological use as, for example, coral probiotics and other natural products. Here, the strategies and technologies that have been used to access cultured CAMs are presented, while advantages and disadvantages associated with each of these strategies are discussed. We highlight the existing gaps and potential improvements in culture-dependent methodologies, indicating several possible alternatives (including culturomics and in situ diffusion devices) that could be applied to retrieve the CAM “dark matter” (i.e., the currently undescribed CAMs). This study provides the most comprehensive synthesis of the methodologies used to recover the cultured coral microbiome to date and draws suggestions for the development of the next generation of CAM culturomics.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SCHULTZ, Júnia, Flúvio MODOLON, Alexandre S. ROSADO, Christian R. VOOLSTRA, Michael SWEET, Raquel S. PEIXOTO, 2022. Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter. In: mSystems. American Society for Microbiology. 2022, 7(4), 00367-22. ISSN 2379-5077. eISSN 2379-5077. Available under: doi: 10.1128/msystems.00367-22
BibTex
@article{Schultz2022-08-30Metho-58126,
  year={2022},
  doi={10.1128/msystems.00367-22},
  title={Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter},
  number={4},
  volume={7},
  issn={2379-5077},
  journal={mSystems},
  author={Schultz, Júnia and Modolon, Flúvio and Rosado, Alexandre S. and Voolstra, Christian R. and Sweet, Michael and Peixoto, Raquel S.},
  note={Article Number: 00367-22}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/58126">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58126/1/Schultz_2-ljyj5ohgc0sf4.pdf"/>
    <dc:creator>Schultz, Júnia</dc:creator>
    <dc:language>eng</dc:language>
    <dc:creator>Voolstra, Christian R.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-07-22T07:22:57Z</dcterms:available>
    <dcterms:abstract xml:lang="eng">The vast majority of environmental microbes have not yet been cultured, and most of the knowledge on coral-associated microbes (CAMs) has been generated from amplicon sequencing and metagenomes. However, exploring cultured CAMs is key for a detailed and comprehensive characterization of the roles of these microbes in shaping coral health and, ultimately, for their biotechnological use as, for example, coral probiotics and other natural products. Here, the strategies and technologies that have been used to access cultured CAMs are presented, while advantages and disadvantages associated with each of these strategies are discussed. We highlight the existing gaps and potential improvements in culture-dependent methodologies, indicating several possible alternatives (including culturomics and in situ diffusion devices) that could be applied to retrieve the CAM “dark matter” (i.e., the currently undescribed CAMs). This study provides the most comprehensive synthesis of the methodologies used to recover the cultured coral microbiome to date and draws suggestions for the development of the next generation of CAM culturomics.</dcterms:abstract>
    <dc:creator>Sweet, Michael</dc:creator>
    <dc:creator>Peixoto, Raquel S.</dc:creator>
    <dcterms:title>Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter</dcterms:title>
    <dc:contributor>Modolon, Flúvio</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-07-22T07:22:57Z</dc:date>
    <dc:contributor>Sweet, Michael</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58126/1/Schultz_2-ljyj5ohgc0sf4.pdf"/>
    <dc:creator>Rosado, Alexandre S.</dc:creator>
    <dc:contributor>Voolstra, Christian R.</dc:contributor>
    <dcterms:issued>2022-08-30</dcterms:issued>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58126"/>
    <dc:contributor>Schultz, Júnia</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Rosado, Alexandre S.</dc:contributor>
    <dc:creator>Modolon, Flúvio</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Peixoto, Raquel S.</dc:contributor>
    <dc:rights>Attribution 4.0 International</dc:rights>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen