An Advanced 'clickECM' that Can be Modified by the Inverse-Electron Demand Diels-Alder Reaction

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2022
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Nellinger, Svenja
Southan, Alexander
Kluger, Petra J.
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Chembiochem. Wiley. 2022, 23(1), e202100266. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.202100266
Zusammenfassung

The extracellular matrix (ECM) represents the natural environment of the cells in tissue and therefore is a promising biomaterial in a variety of applications. Depending on the purpose it is necessary to equip the ECM with specific addressable functional groups for further modification with bioactive molecules, for controllable cross linking and/or covalent binding to surfaces. Metabolic glycoengineering (MGE) enables the specific modification of the ECM with such functional groups without affecting the native structure of the ECM. In a previous approach (S. M. Ruff, S. Keller, D. E. Wieland, V. Wittmann, G. E. M. Tovar, M. Bach, P. J. Kluger, Acta Biomater. 2017 , 52 , 159-170), we demonstrated the modification of an ECM with azido groups which can be addressed by bioorthogonal copper-catalyzed azide-alkyne cycloaddition (CuAAC). Here we demonstrate the modification of an ECM with dienophiles (terminal alkenes, cyclopropene) which can be addressed by an inverse-electron-demand Diels-Alder (IEDDA) reaction. This reaction is cell friendly as there are no cytotoxic catalysts needed. We show the equipment of the ECM with a bioactive molecule (enzyme) and prove the functional groups itself not to influence cellular behavior. Thus, this new material has great potential for its use as biomaterial which can be individually modified in a wide range of applications.

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ISO 690NELLINGER, Svenja, Mareike A. RAPP, Alexander SOUTHAN, Valentin WITTMANN, Petra J. KLUGER, 2022. An Advanced 'clickECM' that Can be Modified by the Inverse-Electron Demand Diels-Alder Reaction. In: Chembiochem. Wiley. 2022, 23(1), e202100266. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.202100266
BibTex
@article{Nellinger2022-01-05Advan-54476,
  year={2022},
  doi={10.1002/cbic.202100266},
  title={An Advanced 'clickECM' that Can be Modified by the Inverse-Electron Demand Diels-Alder Reaction},
  number={1},
  volume={23},
  issn={1439-4227},
  journal={Chembiochem},
  author={Nellinger, Svenja and Rapp, Mareike A. and Southan, Alexander and Wittmann, Valentin and Kluger, Petra J.},
  note={Article Number: e202100266}
}
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