Aptazyme-Mediated Regulation of 16S Ribosomal RNA

Kurzanzeige
dc.contributor.authorWieland, Markus
dc.contributor.authorBerschneider, Barbaradeu
dc.contributor.authorErlacher, Matthias D.deu
dc.contributor.authorHartig, Jörg S.
dc.date.accessioned2011-03-24T18:15:30Zdeu
dc.date.available2011-03-24T18:15:30Zdeu
dc.date.issued2010deu
dc.description.abstractDeveloping artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli. For this purpose, we incorporated hammerhead ribozymes at several positions into orthogonalized 16S rRNA. We observed that ribosomal function is remarkably tolerant toward the incorporation of large additional RNA fragments at certain sites of the 16S rRNA. However, ribozyme-mediated cleavage results in severe reduction of 16S rRNA stability. We carried out an in vivo screen for the identification of sequences acting as ligand-responsive RNA switches, enabling thiamine-dependent switching of 16S rRNA function. In addition to expanding the regulatory toolbox, the presented artificial riboswitches should prove valuable to study aspects of rRNA folding and stability in bacteria.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Chemistry & Biology 17 (2010), 3, pp. 236-242deu
dc.identifier.doi10.1016/j.chembiol.2010.02.012
dc.identifier.pmid20338515
dc.identifier.ppn33759824Xdeu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/9945
dc.language.isoengdeu
dc.legacy.dateIssued2011deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectCHEMBIOdeu
dc.subjectRNAdeu
dc.subject.ddc540deu
dc.titleAptazyme-Mediated Regulation of 16S Ribosomal RNAeng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Wieland2010Aptaz-9945,
  year={2010},
  doi={10.1016/j.chembiol.2010.02.012},
  title={Aptazyme-Mediated Regulation of 16S Ribosomal RNA},
  number={3},
  volume={17},
  issn={1074-5521},
  journal={Chemistry & Biology},
  pages={236--242},
  author={Wieland, Markus and Berschneider, Barbara and Erlacher, Matthias D. and Hartig, Jörg S.}
}
kops.citation.iso690WIELAND, Markus, Barbara BERSCHNEIDER, Matthias D. ERLACHER, Jörg S. HARTIG, 2010. Aptazyme-Mediated Regulation of 16S Ribosomal RNA. In: Chemistry & Biology. 2010, 17(3), pp. 236-242. ISSN 1074-5521. eISSN 1879-1301. Available under: doi: 10.1016/j.chembiol.2010.02.012deu
kops.citation.iso690WIELAND, Markus, Barbara BERSCHNEIDER, Matthias D. ERLACHER, Jörg S. HARTIG, 2010. Aptazyme-Mediated Regulation of 16S Ribosomal RNA. In: Chemistry & Biology. 2010, 17(3), pp. 236-242. ISSN 1074-5521. eISSN 1879-1301. Available under: doi: 10.1016/j.chembiol.2010.02.012eng
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