Insights into the Conformational Plasticity of the Protein Kinase Akt1 by Multi-Lateral Dipolar Spectroscopy

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Chemistry - A European Journal. Wiley. 2023, 29(24), e202203959. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.202203959
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The serine/threonine kinase Akt1 is part of the PI3 K/Akt pathway and plays a key role in the regulation of various cellular processes such as cell growth, proliferation, and apoptosis. Here, we analyzed the elasticity between the two domains of the kinase Akt1, connected by a flexible linker, recording a wide variety of distance restraints by electron paramagnetic resonance (EPR) spectroscopy. We studied full length Akt1 and the influence of the cancer-associated mutation E17K. The conformational landscape in the presence of different modulators, like different types of inhibitors and membranes was presented, revealing a tuned flexibility between the two domains, dependent on the bound molecule.

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ISO 690STEHLE, Juliane, Jörn WEISNER, Leanne EICHHORN, Daniel RAUH, Malte DRESCHER, 2023. Insights into the Conformational Plasticity of the Protein Kinase Akt1 by Multi-Lateral Dipolar Spectroscopy. In: Chemistry - A European Journal. Wiley. 2023, 29(24), e202203959. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.202203959
BibTex
@article{Stehle2023Insig-66452,
  year={2023},
  doi={10.1002/chem.202203959},
  title={Insights into the Conformational Plasticity of the Protein Kinase Akt1 by Multi-Lateral Dipolar Spectroscopy},
  number={24},
  volume={29},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  author={Stehle, Juliane and Weisner, Jörn and Eichhorn, Leanne and Rauh, Daniel and Drescher, Malte},
  note={Article Number: e202203959}
}
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