Publikation:

Retrospective assessment of liver cell proliferation via PCNA : a comparison with tritiated thymidine

Lade...
Vorschaubild

Dateien

Dietrich_199445_51.pdf
Dietrich_199445_51.pdfGröße: 732.28 KBDownloads: 426

Datum

1994

Autor:innen

Candrian, Regula
Marsman, Daniel S.
Popp, James A.
Kaufmann, William K.
Swenberg, James A.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Cancer letters. 1994, 79(1), pp. 45-51. ISSN 0304-3835. Available under: doi: 10.1016/0304-3835(94)90061-2

Zusammenfassung

Cell proliferation (S phase response) in archival liver tissues of partially hepatectomized rats was determined via proliferating cell nuclear antigen (PCNA) immunohistochemistry. These results were compared with the S phase response assessed previously in the same tissues via tritiated thymidine (Tdr) autoradiography. The effect of prolonged tissue fixation on PCNA immunohistochemistry was compared in two studies: study A, the liver was fixed for a maximum of 7 days and then embedded in paraffin and stored for not, vert, similar 18 months, while in study B, the liver was fixed in formalin for 7 years and then embedded in paraffin and stored for not, vert, similar 18 months until sectioning and immunostaining. PCNA immunostaining was successful in the liver sections of both studies, irrespective of the length of formalin fixation. Furthermore, the S phase labeling indices (LI) determined via PCNA and Tdr were comparable, although not identical, in the two studies. Therefore, use of PCNA immunohistochemistry should allow retrospective staining of rodent tissues for the assessment of cell proliferative activity in formalin-fixed organs from previously conducted toxicity and carcinogenicity studies.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

PCNA, Tritiated thymidine, Cell proliferation, Liver, Retrospective

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690DIETRICH, Daniel R., Regula CANDRIAN, Daniel S. MARSMAN, James A. POPP, William K. KAUFMANN, James A. SWENBERG, 1994. Retrospective assessment of liver cell proliferation via PCNA : a comparison with tritiated thymidine. In: Cancer letters. 1994, 79(1), pp. 45-51. ISSN 0304-3835. Available under: doi: 10.1016/0304-3835(94)90061-2
BibTex
@article{Dietrich1994Retro-7133,
  year={1994},
  doi={10.1016/0304-3835(94)90061-2},
  title={Retrospective assessment of liver cell proliferation via PCNA : a comparison with tritiated thymidine},
  number={1},
  volume={79},
  issn={0304-3835},
  journal={Cancer letters},
  pages={45--51},
  author={Dietrich, Daniel R. and Candrian, Regula and Marsman, Daniel S. and Popp, James A. and Kaufmann, William K. and Swenberg, James A.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7133">
    <dc:contributor>Popp, James A.</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Candrian, Regula</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Swenberg, James A.</dc:creator>
    <dc:contributor>Marsman, Daniel S.</dc:contributor>
    <dc:creator>Marsman, Daniel S.</dc:creator>
    <dc:creator>Popp, James A.</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Kaufmann, William K.</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7133/1/Dietrich_199445_51.pdf"/>
    <dcterms:title>Retrospective assessment of liver cell proliferation via PCNA : a comparison with tritiated thymidine</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:43Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7133/1/Dietrich_199445_51.pdf"/>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:43Z</dcterms:available>
    <dc:contributor>Dietrich, Daniel R.</dc:contributor>
    <dc:creator>Kaufmann, William K.</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7133"/>
    <dc:contributor>Swenberg, James A.</dc:contributor>
    <dc:creator>Dietrich, Daniel R.</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:issued>1994</dcterms:issued>
    <dcterms:bibliographicCitation>First publ. in: Cancer letters 79 (1994), 1, pp. 45-51</dcterms:bibliographicCitation>
    <dc:language>eng</dc:language>
    <dc:creator>Candrian, Regula</dc:creator>
    <dcterms:abstract xml:lang="eng">Cell proliferation (S phase response) in archival liver tissues of partially hepatectomized rats was determined via proliferating cell nuclear antigen (PCNA) immunohistochemistry. These results were compared with the S phase response assessed previously in the same tissues via tritiated thymidine (Tdr) autoradiography. The effect of prolonged tissue fixation on PCNA immunohistochemistry was compared in two studies: study A, the liver was fixed for a maximum of 7 days and then embedded in paraffin and stored for not, vert, similar 18 months, while in study B, the liver was fixed in formalin for 7 years and then embedded in paraffin and stored for not, vert, similar 18 months until sectioning and immunostaining. PCNA immunostaining was successful in the liver sections of both studies, irrespective of the length of formalin fixation. Furthermore, the S phase labeling indices (LI) determined via PCNA and Tdr were comparable, although not identical, in the two studies. Therefore, use of PCNA immunohistochemistry should allow retrospective staining of rodent tissues for the assessment of cell proliferative activity in formalin-fixed organs from previously conducted toxicity and carcinogenicity studies.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen