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Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport

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1981

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Bielenberg, Katrin
Spatz, Wolfbernhard B.

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Neuroscience Letters. 1981, 23(1), pp. 1-5. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(81)90181-6

Zusammenfassung

Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB-HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals were identified in the cytoplasm of labeled cells in the proximal portions of the dendrites, and in myelinated axons. It is demonstrated that the TMB-HRP method is well suitable to analyze the ultrastructure of labeled cells and their synaptology.

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570 Biowissenschaften, Biologie

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ISO 690STÜRMER, Claudia, Katrin BIELENBERG, Wolfbernhard B. SPATZ, 1981. Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport. In: Neuroscience Letters. 1981, 23(1), pp. 1-5. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(81)90181-6
BibTex
@article{Sturmer1981Elect-6808,
  year={1981},
  doi={10.1016/0304-3940(81)90181-6},
  title={Electron microscopical identification of 3,3',5,5'-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport},
  number={1},
  volume={23},
  issn={0304-3940},
  journal={Neuroscience Letters},
  pages={1--5},
  author={Stürmer, Claudia and Bielenberg, Katrin and Spatz, Wolfbernhard B.}
}
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