Publikation:

Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum

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2024

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Weiss, Laura
Nymark, Marianne
Roellig, Robert
Wilhelm, Christian

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Deutsche Forschungsgemeinschaft (DFG): KR 1661/19-1
Deutsche Forschungsgemeinschaft (DFG): LE 3358/3-2

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Journal of Experimental Botany. Oxford University Press (OUP). 2024, 75(7), S. 1834-1851. ISSN 0022-0957. eISSN 1460-2431. Verfügbar unter: doi: 10.1093/jxb/erad478

Zusammenfassung

Aureochromes (AUREOs) are unique blue light receptors and transcription factors found only in stramenopile algae. While each of the four AUREOs identified in the diatom Phaeodactylum tricornutum may have a specific function, PtAUREO1a has been shown to have a strong impact on overall gene regulation, when light changes from red to blue light conditions. Despite its significance, the molecular mechanism of PtAUREO1a is largely unexplored. To comprehend the overall process of gene regulation by PtAUREO1a, we conducted a series of in vitro and in vivo experiments, including pull-down assays, yeast one-hybrid experiments, and phenotypical characterization using recombinant PtAUREOs and diatom mutant lines expressing a modified PtAureo1a gene. We describe the distinct light absorption properties of four PtAUREOs and the formation of all combinations of their potential dimers. We demonstrate the capability of PtAUREO1a and 1b to activate the genes, diatom-specific cyclin 2, PtAureo1a, and PtAureo1c under both light and dark conditions. Using mutant lines expressing a modified PtAUREO1a protein with a considerably reduced light absorption, we found novel evidence that PtAUREO1a regulates the expression of PtLHCF15, which is essential for red light acclimation. Based on current knowledge, we present a working model of PtAUREO1a gene regulation properties.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Aureochromes, blue light receptors, diatoms, Phaedactylum tricornutum, protein-protein interactions, red-shifted antenna complex, yeast one-hybrid

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ISO 690IM, Soo Hyun, Bernard LEPETIT, Niccolo MOSESSO, Sandeep SHRESTHA, Laura WEISS, Marianne NYMARK, Robert ROELLIG, Christian WILHELM, Erika ISONO, Peter G. KROTH, 2024. Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum. In: Journal of Experimental Botany. Oxford University Press (OUP). 2024, 75(7), S. 1834-1851. ISSN 0022-0957. eISSN 1460-2431. Verfügbar unter: doi: 10.1093/jxb/erad478
BibTex
@article{Im2024-03-27Ident-68998,
  year={2024},
  doi={10.1093/jxb/erad478},
  title={Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum},
  number={7},
  volume={75},
  issn={0022-0957},
  journal={Journal of Experimental Botany},
  pages={1834--1851},
  author={Im, Soo Hyun and Lepetit, Bernard and Mosesso, Niccolo and Shrestha, Sandeep and Weiss, Laura and Nymark, Marianne and Roellig, Robert and Wilhelm, Christian and Isono, Erika and Kroth, Peter G.}
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