Publikation: Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
DENG, Wu-Min, Martina SCHNEIDER, Richard FROCK, Casimiro CASTILLEJO-LOPEZ, Emily Anne GAMAN, Stefan BAUMGARTNER, Hannele RUOHOLA-BAKER, 2003. Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. In: Development. Company of Biologists. 2003, 130(1), pp. 173-184. ISSN 0950-1991. eISSN 1477-9129. Available under: doi: 10.1242/dev.00199BibTex
@article{Deng2003-01Dystr-50883, year={2003}, doi={10.1242/dev.00199}, title={Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila}, number={1}, volume={130}, issn={0950-1991}, journal={Development}, pages={173--184}, author={Deng, Wu-Min and Schneider, Martina and Frock, Richard and Castillejo-Lopez, Casimiro and Gaman, Emily Anne and Baumgartner, Stefan and Ruohola-Baker, Hannele} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/50883"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-18T11:47:46Z</dc:date> <dc:contributor>Schneider, Martina</dc:contributor> <dc:contributor>Castillejo-Lopez, Casimiro</dc:contributor> <dc:creator>Deng, Wu-Min</dc:creator> <dc:creator>Schneider, Martina</dc:creator> <dc:creator>Baumgartner, Stefan</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Frock, Richard</dc:creator> <dc:creator>Castillejo-Lopez, Casimiro</dc:creator> <dc:contributor>Gaman, Emily Anne</dc:contributor> <dcterms:issued>2003-01</dcterms:issued> <dcterms:title>Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila</dcterms:title> <dc:creator>Ruohola-Baker, Hannele</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Ruohola-Baker, Hannele</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Deng, Wu-Min</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Baumgartner, Stefan</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dcterms:abstract xml:lang="eng">The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.</dcterms:abstract> <dc:contributor>Frock, Richard</dc:contributor> <dc:creator>Gaman, Emily Anne</dc:creator> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-18T11:47:46Z</dcterms:available> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50883"/> </rdf:Description> </rdf:RDF>