Publikation:

Exocrine gland–resident memory CD8+ T cells use mechanosensing for tissue surveillance

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2023

Autor:innen

Ruef, Nora
Martínez Magdaleno, Jose
Ficht, Xenia
Palayret, Matthieu
Wissmann, Stefanie
Pfenninger, Petra
Stolp, Bettina
Stein, Jens V.
et al.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Deutsche Forschungsgemeinschaft (DFG): 240245660

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Science Immunology. American Association for the Advancement of Science (AAAS). 2023, 8(90), eadd5724. eISSN 2470-9468. Available under: doi: 10.1126/sciimmunol.add5724

Zusammenfassung

Tissue-resident CD8+ T cells (TRM) continuously scan peptide-MHC (pMHC) complexes in their organ of residence to intercept microbial invaders. Recent data showed that TRM lodged in exocrine glands scan tissue in the absence of any chemoattractant or adhesion receptor signaling, thus bypassing the requirement for canonical migration-promoting factors. The signals eliciting this noncanonical motility and its relevance for organ surveillance have remained unknown. Using mouse models of viral infections, we report that exocrine gland TRM autonomously generated front-to-back F-actin flow for locomotion, accompanied by high cortical actomyosin contractility, and leading-edge bleb formation. The distinctive mode of exocrine gland TRM locomotion was triggered by sensing physical confinement and was closely correlated with nuclear deformation, which acts as a mechanosensor via an arachidonic acid and Ca2+ signaling pathway. By contrast, naïve CD8+ T cells or TRM surveilling microbe-exposed epithelial barriers did not show mechanosensing capacity. Inhibition of nuclear mechanosensing disrupted exocrine gland T RM scanning and impaired their ability to intercept target cells. These findings indicate that confinement is sufficient to elicit autonomous T cell surveillance in glands with restricted chemokine expression and constitutes a scanning strategy that complements chemosensing-dependent migration.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690RUEF, Nora, Jose MARTÍNEZ MAGDALENO, Xenia FICHT, Vladimir PURVANOV, Matthieu PALAYRET, Stefanie WISSMANN, Petra PFENNINGER, Bettina STOLP, Daniel F. LEGLER, Jens V. STEIN, 2023. Exocrine gland–resident memory CD8+ T cells use mechanosensing for tissue surveillance. In: Science Immunology. American Association for the Advancement of Science (AAAS). 2023, 8(90), eadd5724. eISSN 2470-9468. Available under: doi: 10.1126/sciimmunol.add5724
BibTex
@article{Ruef2023-12-22Exocr-69160,
  year={2023},
  doi={10.1126/sciimmunol.add5724},
  title={Exocrine gland–resident memory CD8<sup>+</sup> T cells use mechanosensing for tissue surveillance},
  number={90},
  volume={8},
  journal={Science Immunology},
  author={Ruef, Nora and Martínez Magdaleno, Jose and Ficht, Xenia and Purvanov, Vladimir and Palayret, Matthieu and Wissmann, Stefanie and Pfenninger, Petra and Stolp, Bettina and Legler, Daniel F. and Stein, Jens V.},
  note={Article Number: eadd5724}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/69160">
    <dc:contributor>Palayret, Matthieu</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/69160"/>
    <dc:creator>Stolp, Bettina</dc:creator>
    <dc:contributor>Legler, Daniel F.</dc:contributor>
    <dc:creator>Wissmann, Stefanie</dc:creator>
    <dc:contributor>Stolp, Bettina</dc:contributor>
    <dcterms:issued>2023-12-22</dcterms:issued>
    <dc:contributor>Ruef, Nora</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Wissmann, Stefanie</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-01-23T07:42:57Z</dc:date>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-01-23T07:42:57Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Ruef, Nora</dc:creator>
    <dc:contributor>Ficht, Xenia</dc:contributor>
    <dc:contributor>Stein, Jens V.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Legler, Daniel F.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Martínez Magdaleno, Jose</dc:creator>
    <dc:contributor>Martínez Magdaleno, Jose</dc:contributor>
    <dc:creator>Ficht, Xenia</dc:creator>
    <dcterms:title>Exocrine gland–resident memory CD8&lt;sup&gt;+&lt;/sup&gt; T cells use mechanosensing for tissue surveillance</dcterms:title>
    <dc:creator>Pfenninger, Petra</dc:creator>
    <dcterms:abstract>Tissue-resident CD8&lt;sup&gt;+&lt;/sup&gt; T cells (T&lt;sub&gt;RM&lt;/sub&gt;) continuously scan peptide-MHC (pMHC) complexes in their organ of residence to intercept microbial invaders. Recent data showed that T&lt;sub&gt;RM&lt;/sub&gt; lodged in exocrine glands scan tissue in the absence of any chemoattractant or adhesion receptor signaling, thus bypassing the requirement for canonical migration-promoting factors. The signals eliciting this noncanonical motility and its relevance for organ surveillance have remained unknown. Using mouse models of viral infections, we report that exocrine gland T&lt;sub&gt;RM&lt;/sub&gt; autonomously generated front-to-back F-actin flow for locomotion, accompanied by high cortical actomyosin contractility, and leading-edge bleb formation. The distinctive mode of exocrine gland T&lt;sub&gt;RM&lt;/sub&gt; locomotion was triggered by sensing physical confinement and was closely correlated with nuclear deformation, which acts as a mechanosensor via an arachidonic acid and Ca&lt;sup&gt;2+&lt;/sup&gt; signaling pathway. By contrast, naïve CD8&lt;sup&gt;+&lt;/sup&gt; T cells or T&lt;sub&gt;RM&lt;/sub&gt; surveilling microbe-exposed epithelial barriers did not show mechanosensing capacity. Inhibition of nuclear mechanosensing disrupted exocrine gland T       RM       scanning and impaired their ability to intercept target cells. These findings indicate that confinement is sufficient to elicit autonomous T cell surveillance in glands with restricted chemokine expression and constitutes a scanning strategy that complements chemosensing-dependent migration.</dcterms:abstract>
    <dc:creator>Stein, Jens V.</dc:creator>
    <dc:contributor>Pfenninger, Petra</dc:contributor>
    <dc:creator>Purvanov, Vladimir</dc:creator>
    <dc:creator>Palayret, Matthieu</dc:creator>
    <dc:contributor>Purvanov, Vladimir</dc:contributor>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen