Identification of MAC1 : A Small Molecule That Rescues Spindle Bipolarity in Monastrol-Treated Cells

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2016
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Mitchison, Timothy J.
Crews, Craig M.
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The genetic integrity of each organism is intimately tied to the correct segregation of its genome during mitosis. Insights into the underlying mechanisms are fundamental for both basic research and the development of novel strategies to treat mitosis-relevant diseases such as cancer. Due to their fast mode of action, small molecules are invaluable tools to dissect mitosis. Yet, there is a great demand for novel antimitotic compounds. We performed a chemical genetic suppression screen to identify compounds that restore spindle bipolarity in cells treated with Monastrol, an inhibitor of the mitotic kinesin Eg5. We identified one compound-MAC1-that rescued spindle bipolarity in cells lacking Eg5 activity. Mechanistically, MAC1 induces the formation of additional microtubule nucleation centers, which allows kinesin Kif15-dependent bipolar spindle assembly in the absence of Eg5 activity. Thus, our chemical genetic suppression screen revealed novel unexpected insights into the mechanism of spindle assembly in mammalian cells.

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ISO 690AL-OBAIDI, Naowras, Timothy J. MITCHISON, Craig M. CREWS, Thomas U. MAYER, 2016. Identification of MAC1 : A Small Molecule That Rescues Spindle Bipolarity in Monastrol-Treated Cells. In: ACS Chemical Biology. 2016, 11(6), pp. 1544-1551. ISSN 1554-8929. eISSN 1554-8937. Available under: doi: 10.1021/acschembio.6b00203
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@article{AlObaidi2016-06-17Ident-35225,
  year={2016},
  doi={10.1021/acschembio.6b00203},
  title={Identification of MAC1 : A Small Molecule That Rescues Spindle Bipolarity in Monastrol-Treated Cells},
  number={6},
  volume={11},
  issn={1554-8929},
  journal={ACS Chemical Biology},
  pages={1544--1551},
  author={Al-Obaidi, Naowras and Mitchison, Timothy J. and Crews, Craig M. and Mayer, Thomas U.}
}
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    <dcterms:abstract xml:lang="eng">The genetic integrity of each organism is intimately tied to the correct segregation of its genome during mitosis. Insights into the underlying mechanisms are fundamental for both basic research and the development of novel strategies to treat mitosis-relevant diseases such as cancer. Due to their fast mode of action, small molecules are invaluable tools to dissect mitosis. Yet, there is a great demand for novel antimitotic compounds. We performed a chemical genetic suppression screen to identify compounds that restore spindle bipolarity in cells treated with Monastrol, an inhibitor of the mitotic kinesin Eg5. We identified one compound-MAC1-that rescued spindle bipolarity in cells lacking Eg5 activity. Mechanistically, MAC1 induces the formation of additional microtubule nucleation centers, which allows kinesin Kif15-dependent bipolar spindle assembly in the absence of Eg5 activity. Thus, our chemical genetic suppression screen revealed novel unexpected insights into the mechanism of spindle assembly in mammalian cells.</dcterms:abstract>
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