Ion Selectivity of the cytoplasmic binding sites of the Na,K-ATPase : I. sodium binding is associated with a conformational rearrangement
Ion Selectivity of the cytoplasmic binding sites of the Na,K-ATPase : I. sodium binding is associated with a conformational rearrangement
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1999
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Schneeberger, Anne
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Journal of Membrane Biology ; 168 (1999), 3. - pp. 221-228. - ISSN 0022-2631. - eISSN 1432-1424
Abstract
To investigate Na+ binding to the ion-binding sites presented on the cytoplasmic side of the Na,KATPase, equilibrium Na+-titration experiments were performed using two fluorescent dyes, RH421¹ and FITC, to detect protein-specific actions. Fluorescence changes upon addition of Na+ in the presence of various Mg2+ concentrations were similar and could be fitted with a Hill function. The half-saturating concentrations and Hill coefficients determined were almost identical. As RH421 responds to binding of a Na+ ion to the third neutral site whereas FITC monitors conformational changes in the ATP-binding site or its environment, this result implies that electrogenic binding of the third Na+ ion is the trigger for a structural rearrangement of the ATP-binding moiety. This enables enzyme phosphorylation, which is accompanied by a fast occlusion of the Na+ ions and followed by the conformational transition E1/E2 of the protein. The coordinated action both at the ion and the nucleotide binding sites allows for the first time a detailed formulation of the mechanism of enzyme phosphorylation that occurs only when three Na+ ions are bound.
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570 Biosciences, Biology
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Na,K-ATPase,Cytoplasmic ion binding,Electrochromic fluorescent dye,FITC,Ion transport,Energy transduction mechanism
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SCHNEEBERGER, Anne, Hans-Jürgen APELL, 1999. Ion Selectivity of the cytoplasmic binding sites of the Na,K-ATPase : I. sodium binding is associated with a conformational rearrangement. In: Journal of Membrane Biology. 168(3), pp. 221-228. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002329900511BibTex
@article{Schneeberger1999Selec-7625, year={1999}, doi={10.1007/s002329900511}, title={Ion Selectivity of the cytoplasmic binding sites of the Na,K-ATPase : I. sodium binding is associated with a conformational rearrangement}, number={3}, volume={168}, issn={0022-2631}, journal={Journal of Membrane Biology}, pages={221--228}, author={Schneeberger, Anne and Apell, Hans-Jürgen} }
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