Mass Spectrometric Analysis of Noncovalent Complexes Between Synthetic Peptides from Human Ribosomal Protein L7 and Protein G

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2015
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Ma, Li
Kohlmann, Markus
Wochner, Michael
Krawinkel, Ulrich
Liu, Shuying
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In order to gain a comprehensive insight into the complexes of human ribosomal protein L7 with protein G in a certain degree, an investigation on the complexes of five synthetic L7 peptides, containing the basic-region-leucine-zipper (BZIP)-like domain (aa 15–49), with protein G was performed using nanoelectrospray ionization mass spectrometry (nanoESI-MS). Circular dichroism (CD) was used to characterize the secondary structures of L7 peptides. The characteristics of the complexes between L7 peptides and protein G were studied under various conditions, such as molar ratio of ligands, solvent condition, declustering potential, and peptide sequence. The stability of the complexes is found to decrease with increased declustering potential (>20 V), decreased pH (<5), increased pH (>5), while L7 peptide sequence had no obvious effect on the complex formation. Taken together, the complexes of L7 peptides with protein G are specific noncovalent binding with 1:1 stoichiometry. Because of the availability of synthetic L7 peptides, they might be used as baits to discover the binding partners of protein L7. Furthermore, the elaboration of the binding mechanisms of L7 peptides with protein G could benefit further application of protein G.

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540 Chemie
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electronspray ionization, L7 peptide, mass spectrometry, noncovalent complex, protein G, stability
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ISO 690MA, Li, Markus KOHLMANN, Michael WOCHNER, Ulrich KRAWINKEL, Michael PRZYBYLSKI, Shuying LIU, 2015. Mass Spectrometric Analysis of Noncovalent Complexes Between Synthetic Peptides from Human Ribosomal Protein L7 and Protein G. In: Journal of Liquid Chromatography & Related Technologies. 2015, 38(10), pp. 1007-1013. ISSN 0148-3919. eISSN 1520-572X. Available under: doi: 10.1080/10826076.2014.922475
BibTex
@article{Ma2015Spect-31101,
  year={2015},
  doi={10.1080/10826076.2014.922475},
  title={Mass Spectrometric Analysis of Noncovalent Complexes Between Synthetic Peptides from Human Ribosomal Protein L7 and Protein G},
  number={10},
  volume={38},
  issn={0148-3919},
  journal={Journal of Liquid Chromatography & Related Technologies},
  pages={1007--1013},
  author={Ma, Li and Kohlmann, Markus and Wochner, Michael and Krawinkel, Ulrich and Przybylski, Michael and Liu, Shuying}
}
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