The Deubiquitinating Enzyme AMSH3 Is Required for Intracellular Trafficking and Vacuole Biogenesis in Arabidopsis thaliana

Isono, Erika; Katsiarimpa, Anthi; Müller, Isabel Karin; Anzenberger, Franziska; Stierhof, York-Dieter; Geldner, Niko; Chory, Joanne; Schwechheimer, Claus

The Deubiquitinating Enzyme AMSH3 Is Required for Intracellular Trafficking and Vacuole Biogenesis in Arabidopsis thaliana

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Date

2010

Authors

Isono, Erika

Katsiarimpa, Anthi

Müller, Isabel Karin

Anzenberger, Franziska

Stierhof, York-Dieter

Geldner, Niko

Chory, Joanne

Schwechheimer, Claus

DOI (citable link)

10.1105/tpc.110.075952

Collections

Biologie

Publication type

Journal article

Publication status

Published

Published in

The Plant Cell ; 22 (2010), 6. - pp. 1826-1837. - ISSN 1040-4651. - eISSN 1532-298X

Abstract

Ubiquitination, deubiquitination, and the formation of specific ubiquitin chain topologies have been implicated in various cellular processes. Little is known, however, about the role of ubiquitin in the development of cellular organelles. Here, we identify and characterize the deubiquitinating enzyme AMSH3 from Arabidopsis thaliana. AMSH3 hydrolyzes K48- and K63-linked ubiquitin chains in vitro and accumulates both ubiquitin chain types in vivo. amsh3 mutants fail to form a central lytic vacuole, accumulate autophagosomes, and mis-sort vacuolar protein cargo to the intercellular space. Furthermore, AMSH3 is required for efficient endocytosis of the styryl dye FM4-64 and the auxin efflux facilitator PIN2. We thus present evidence for a role of deubiquitination in intracellular trafficking and vacuole biogenesis.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

ISONO, Erika, Anthi KATSIARIMPA, Isabel Karin MÜLLER, Franziska ANZENBERGER, York-Dieter STIERHOF, Niko GELDNER, Joanne CHORY, Claus SCHWECHHEIMER, 2010. The Deubiquitinating Enzyme AMSH3 Is Required for Intracellular Trafficking and Vacuole Biogenesis in Arabidopsis thaliana. In: The Plant Cell. 22(6), pp. 1826-1837. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.110.075952

BibTex

@article{Isono2010-06Deubi-42124,
  year={2010},
  doi={10.1105/tpc.110.075952},
  title={The Deubiquitinating Enzyme AMSH3 Is Required for Intracellular Trafficking and Vacuole Biogenesis in Arabidopsis thaliana},
  number={6},
  volume={22},
  issn={1040-4651},
  journal={The Plant Cell},
  pages={1826--1837},
  author={Isono, Erika and Katsiarimpa, Anthi and Müller, Isabel Karin and Anzenberger, Franziska and Stierhof, York-Dieter and Geldner, Niko and Chory, Joanne and Schwechheimer, Claus}
}

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