Binding of Potassium Ions Inside the Access Channel at the Cytoplasmic Side of Na+,K+-ATPase

Thumbnail Image
Files
Vishnyakova_2-edjyr3zpzdkj4.pdf(134.45 KB)
Date
2018
Authors
Vishnyakova, V. Ye.
Tashkin, Vsevolod Yu.
Terentjev, A. O.
Sokolov, Valeriy S.
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
urn:nbn:de:bsz:352-2-edjyr3zpzdkj4
DOI (citable link)
10.1134/S1990747818050082
ArXiv-ID
International patent number
Link to the license
In Copyright
EU project number
Project
Open Access publication
Collections
Biologie
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published
Published in
Biochemistry (Moscow), Supplement Series A : Membrane and Cell Biology ; 12 (2018), 4. - pp. 344-351. - ISSN 1990-7478. - eISSN 1990-7494
Abstract
Binding of potassium ions through an access channel from the cytoplasmic side of Na+,K+-ATPase and the effect of pH and magnesium ions on this process have been studied. The studies were carried out by a previously developed method of measuring small increments of the admittance (capacitance and conductivity) of a compound membrane consisting of a bilayer lipid membrane with adsorbed membranes fragments containing Na+,K+-ATPase. The capacitance change of the membrane with the Na+,K+-ATPase was induced abruptly by release of protons from a bound form (caged H+) upon a UV-light flash in the absence of magnesium ions. The change of admittance consisted of an initial fast jump and a slow relaxation to a stationary value within a time of about 1–2 s. The kinetics of the capacitance relaxation depended on pH and the concentration of magnesium and potassium ions. The dependence of the rapid capacitance jump on the potassium concentration corresponded to the predictions of the model developed earlier that describes binding of sodium or potassium ions in competition with protons. The effect of magnesium ions can be explained by the assuming that they bind to the Na+,K+-ATPase and affect binding of potassium ions because of either changes in protein conformation or the creation of an electrostatic field in the access channel on the cytoplasmic side.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Na+,K+-ATPase, sodium pump, electrogenic ion transport, caged proton, admittance measurement, K+ binding by the Na+,K+-ATPase
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690VISHNYAKOVA, V. Ye., Vsevolod Yu. TASHKIN, A. O. TERENTJEV, Hans-Jürgen APELL, Valeriy S. SOKOLOV, 2018. Binding of Potassium Ions Inside the Access Channel at the Cytoplasmic Side of Na+,K+-ATPase. In: Biochemistry (Moscow), Supplement Series A : Membrane and Cell Biology. 12(4), pp. 344-351. ISSN 1990-7478. eISSN 1990-7494. Available under: doi: 10.1134/S1990747818050082
BibTex
@article{Vishnyakova2018-12-25Bindi-45638,
  year={2018},
  doi={10.1134/S1990747818050082},
  title={Binding of Potassium Ions Inside the Access Channel at the Cytoplasmic Side of Na<sup>+</sup>,K<sup>+</sup>-ATPase},
  number={4},
  volume={12},
  issn={1990-7478},
  journal={Biochemistry (Moscow), Supplement Series A : Membrane and Cell Biology},
  pages={344--351},
  author={Vishnyakova, V. Ye. and Tashkin, Vsevolod Yu. and Terentjev, A. O. and Apell, Hans-Jürgen and Sokolov, Valeriy S.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/45638">
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Apell, Hans-Jürgen</dc:contributor>
    <dc:contributor>Tashkin, Vsevolod Yu.</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/45638"/>
    <dc:creator>Tashkin, Vsevolod Yu.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-04-15T13:07:16Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-04-15T13:07:16Z</dc:date>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:issued>2018-12-25</dcterms:issued>
    <dc:contributor>Sokolov, Valeriy S.</dc:contributor>
    <dcterms:title>Binding of Potassium Ions Inside the Access Channel at the Cytoplasmic Side of Na&lt;sup&gt;+&lt;/sup&gt;,K&lt;sup&gt;+&lt;/sup&gt;-ATPase</dcterms:title>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Apell, Hans-Jürgen</dc:creator>
    <dc:contributor>Vishnyakova, V. Ye.</dc:contributor>
    <dc:contributor>Terentjev, A. O.</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/45638/3/Vishnyakova_2-edjyr3zpzdkj4.pdf"/>
    <dc:creator>Sokolov, Valeriy S.</dc:creator>
    <dc:creator>Vishnyakova, V. Ye.</dc:creator>
    <dcterms:abstract xml:lang="eng">Binding of potassium ions through an access channel from the cytoplasmic side of Na+,K+-ATPase and the effect of pH and magnesium ions on this process have been studied. The studies were carried out by a previously developed method of measuring small increments of the admittance (capacitance and conductivity) of a compound membrane consisting of a bilayer lipid membrane with adsorbed membranes fragments containing Na&lt;sup&gt;+&lt;/sup&gt;,K&lt;sup&gt;+&lt;/sup&gt;-ATPase. The capacitance change of the membrane with the Na&lt;sup&gt;+&lt;/sup&gt;,K&lt;sup&gt;+&lt;/sup&gt;-ATPase was induced abruptly by release of protons from a bound form (caged H+) upon a UV-light flash in the absence of magnesium ions. The change of admittance consisted of an initial fast jump and a slow relaxation to a stationary value within a time of about 1–2 s. The kinetics of the capacitance relaxation depended on pH and the concentration of magnesium and potassium ions. The dependence of the rapid capacitance jump on the potassium concentration corresponded to the predictions of the model developed earlier that describes binding of sodium or potassium ions in competition with protons. The effect of magnesium ions can be explained by the assuming that they bind to the Na&lt;sup&gt;+&lt;/sup&gt;,K&lt;sup&gt;+&lt;/sup&gt;-ATPase and affect binding of potassium ions because of either changes in protein conformation or the creation of an electrostatic field in the access channel on the cytoplasmic side.</dcterms:abstract>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/45638/3/Vishnyakova_2-edjyr3zpzdkj4.pdf"/>
    <dc:creator>Terentjev, A. O.</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed
Unknown