Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards

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2018
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European Union (EU): 614606
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I-SURF : Inorganic surfactants with multifunctinal heads
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ACS applied materials & interfaces. 2018, 10(28), pp. 23638-23646. ISSN 1944-8244. eISSN 1944-8252. Available under: doi: 10.1021/acsami.8b07032
Zusammenfassung

The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical purposes, for example, imaging, to learn something about intracellular processes. Cases describing the improvement of a biological function by the integration of a nonnatural (nano)constituent are extremely rare. Because biological membranes contain some kind of a surfactant, for example, phospholipids, our idea is to modify cells with a newly synthesized surfactant. However, this surfactant is intended to possess an additional functionality, which is the reduction of oxidative stress. We report the synthesis of a surfactant with Janus-type head group architecture, a fullerene C60 modified by five alkyl chains on one side and an average of 20 oxygen species on the other hemisphere. It is demonstrated that the amphiphilic properties of the fullerenol surfactant are similar to that of lipids. Not only quenching of reactive oxygen species (superoxide, hydroxyl radicals, peroxynitrite, and hydrogen peroxide) was successful, but also the fullerenol surfactant exceeds benchmark antioxidant agents such as quercetin. The surfactant was then brought into contact with different cell types, and the viability even of delicate cells such as human liver cells (HepG2) and human dopaminergic neurons (LUHMES) has proven to be extraordinarily high. We could show further that the cells take up the fullerenol surfactant, and as a consequence, they are protected much better against oxidative stress.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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amphiphile; fullerene; fullerenol; living cells; membrane; ROS quenching; superoxide; surfactant
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ISO 690KUNKEL, Marius, Stefan SCHILDKNECHT, Klaus BOLDT, Lukas ZEYFFERT, David SCHLEHECK, Marcel LEIST, Sebastian POLARZ, 2018. Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards. In: ACS applied materials & interfaces. 2018, 10(28), pp. 23638-23646. ISSN 1944-8244. eISSN 1944-8252. Available under: doi: 10.1021/acsami.8b07032
BibTex
@article{Kunkel2018-07-18Incre-42814,
  year={2018},
  doi={10.1021/acsami.8b07032},
  title={Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards},
  number={28},
  volume={10},
  issn={1944-8244},
  journal={ACS applied materials & interfaces},
  pages={23638--23646},
  author={Kunkel, Marius and Schildknecht, Stefan and Boldt, Klaus and Zeyffert, Lukas and Schleheck, David and Leist, Marcel and Polarz, Sebastian}
}
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