Optical microscopy measurement of pair correlation functions

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2006
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Ramírez-Saito, Angeles
Arauz-Lara, José Luis
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We studied the pair correlation function g(r) of silica particles with a fluorescent core and a nonfluorescent shell which were confined between two glass plates by optical video microscopy. To investigate the possible role of optical artifacts due to overlapping particle images, we compared experiments, where, first, the whole particle (white image) and then, only the fluorescent core (fluorescent image) was used for determining particle positions. While under white-image conditions the observed g(r) exhibits a main peak at about 1.2 times the particle's diameter; under fluorescent image conditions the obtained g(r) resembles a short-ranged repulsive system where the main peak is close to contact. This discrepancy points towards artifacts of video microscopy, leading to erroneous g(r) and in turn to erroneous effective-pair potentials.

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ISO 690RAMÍREZ-SAITO, Angeles, Clemens BECHINGER, José Luis ARAUZ-LARA, 2006. Optical microscopy measurement of pair correlation functions. In: Physical Review E. 2006, 74(3), 030401. ISSN 1539-3755. eISSN 1550-2376. Available under: doi: 10.1103/PhysRevE.74.030401
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@article{RamirezSaito2006-09-01Optic-39211,
  year={2006},
  doi={10.1103/PhysRevE.74.030401},
  title={Optical microscopy measurement of pair correlation functions},
  number={3},
  volume={74},
  issn={1539-3755},
  journal={Physical Review E},
  author={Ramírez-Saito, Angeles and Bechinger, Clemens and Arauz-Lara, José Luis},
  note={Article Number: 030401}
}
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