Publikation:

Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence

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2018

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Schindler, Torben
González, Adrián
Boopathi, Ramachandran
Roda, Marta Marty
Romero-Santacreu, Lorena
Wildes, Andrew
Porcar, Lionel
Martel, Anne
Peyrard, Michel
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https://doi.org/10.1103/PhysRevE.98.042417
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http://arxiv.org/abs/1809.09335

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Physical Review E. 2018, 98(4), 042417. ISSN 2470-0045. eISSN 2470-0053. Available under: doi: 10.1103/PhysRevE.98.042417

Zusammenfassung

DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of lp≈500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain.

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530 Physik

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ISO 690SCHINDLER, Torben, Adrián GONZÁLEZ, Ramachandran BOOPATHI, Marta Marty RODA, Lorena ROMERO-SANTACREU, Andrew WILDES, Lionel PORCAR, Anne MARTEL, Nikos THEODORAKOPOULOS, Michel PEYRARD, 2018. Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence. In: Physical Review E. 2018, 98(4), 042417. ISSN 2470-0045. eISSN 2470-0053. Available under: doi: 10.1103/PhysRevE.98.042417
BibTex
@article{Schindler2018Kinky-45161,
  year={2018},
  doi={10.1103/PhysRevE.98.042417},
  title={Kinky DNA in solution : Small-angle-scattering study of a nucleosome positioning sequence},
  number={4},
  volume={98},
  issn={2470-0045},
  journal={Physical Review E},
  author={Schindler, Torben and González, Adrián and Boopathi, Ramachandran and Roda, Marta Marty and Romero-Santacreu, Lorena and Wildes, Andrew and Porcar, Lionel and Martel, Anne and Theodorakopoulos, Nikos and Peyrard, Michel},
  note={Article Number: 042417}
}
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