Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody

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1992
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Pircher, Hanspeter
Rebaï, Najet
Grégoire, Claude
Speiser, Daniel E.
Happ, Mary Patt
Palmer, Ed
Zinkernagel, Rolf M.
Hengartner, Hans
Malissen, Bernard
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A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specific for murine TcR V alpha 2 chains. The V alpha 2+ T cell population was examined in various inbred strains by two-color immunofluorescence using B20.1 and CD4- and CD8-specific antibodies with the following results: (a) the B20.1 antibody detected most members of the TcR V alpha 2 subfamily in the four TcR V alpha haplotypes tested; (b) in most strains examined, TcR V alpha 2 expression was biased to the CD4 subset (7.4%-17.4% V alpha 2+ T cells) as compared to the CD8 compartment (3.8%-13.3%); (c) TcR V alpha 2 expression was not influenced by Mls gene products and (d) increased positive selection of V alpha 2+ CD8+ T cells by H-2k major histocompatibility complex molecules occurred in all murine strains tested of the TcR V alpha a, but not in those bearing the TcR V alpha b haplotype.

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570 Biowissenschaften, Biologie
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ISO 690PIRCHER, Hanspeter, Najet REBAÏ, Marcus GRÖTTRUP, Claude GRÉGOIRE, Daniel E. SPEISER, Mary Patt HAPP, Ed PALMER, Rolf M. ZINKERNAGEL, Hans HENGARTNER, Bernard MALISSEN, 1992. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody. In: European journal of immunology. 1992, 22(2), pp. 399-404. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/eji.1830220217
BibTex
@article{Pircher1992Prefe-38642,
  year={1992},
  doi={10.1002/eji.1830220217},
  title={Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody},
  number={2},
  volume={22},
  issn={0014-2980},
  journal={European journal of immunology},
  pages={399--404},
  author={Pircher, Hanspeter and Rebaï, Najet and Gröttrup, Marcus and Grégoire, Claude and Speiser, Daniel E. and Happ, Mary Patt and Palmer, Ed and Zinkernagel, Rolf M. and Hengartner, Hans and Malissen, Bernard}
}
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    <dcterms:abstract xml:lang="eng">A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specific for murine TcR V alpha 2 chains. The V alpha 2+ T cell population was examined in various inbred strains by two-color immunofluorescence using B20.1 and CD4- and CD8-specific antibodies with the following results: (a) the B20.1 antibody detected most members of the TcR V alpha 2 subfamily in the four TcR V alpha haplotypes tested; (b) in most strains examined, TcR V alpha 2 expression was biased to the CD4 subset (7.4%-17.4% V alpha 2+ T cells) as compared to the CD8 compartment (3.8%-13.3%); (c) TcR V alpha 2 expression was not influenced by Mls gene products and (d) increased positive selection of V alpha 2+ CD8+ T cells by H-2k major histocompatibility complex molecules occurred in all murine strains tested of the TcR V alpha a, but not in those bearing the TcR V alpha b haplotype.</dcterms:abstract>
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