Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium

Friedrich, Michael; Schink, Bernhard

Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium

Files

Fermentative_degradation_of_glyoxylate_1991.pdf(775.28 KB)

Date

1991

Authors

Friedrich, Michael

Schink, Bernhard

Publication type

Journal article

Published in

Archives of Microbiology ; 156 (1991), 5. - pp. 392-397. - ISSN 0302-8933. - eISSN 1432-072X

Abstract

A new strictly anaerobic, gram-negative, nonsporeforming bacterium, Strain PerGlxl, was enriched and isolated from marine sediment samples with glyoxylate as sole carbon and energy source. The guanineplus-cytosine content of the DNA was 44.1 +/-0.2 mol %. Glyoxylate was utilized as the only substrate and was stoichiometrically degraded to carbon dioxide, hydrogen, and glycolate. An acetyl-CoA and ADP-dependent glyoxylate converting enzyme activity, malic enzyme, and pyruvate synthase were found at activities sufficient for growth (>/= 0.25 U x mg protein- 1). These findings allow to design a new degradation pathway for glyoxylate: glyoxylate is condensed with acetyl-CoA to form malyl-CoA; the free energy of the thioester linkage in malyl-CoA is conserved by substrate level phosphorylation. Part of the electrons released during glyoxylate oxidation to CO2 reduce a small fraction of glyoxylate to glycolate.

Subject (DDC)

570 Biosciences, Biology

Keywords

Glyoxylate,Anaerobic degradation,Malic enzyme,Substrate level phosphorylation

Cite This

ISO 690

FRIEDRICH, Michael, Bernhard SCHINK, 1991. Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium. In: Archives of Microbiology. 156(5), pp. 392-397. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248716

BibTex

@article{Friedrich1991Ferme-7523,
  year={1991},
  doi={10.1007/BF00248716},
  title={Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium},
  number={5},
  volume={156},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={392--397},
  author={Friedrich, Michael and Schink, Bernhard}
}

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