Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium

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Fermentative_degradation_of_glyoxylate_1991.pdf
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1991
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Friedrich, Michael
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Zusammenfassung

A new strictly anaerobic, gram-negative, nonsporeforming bacterium, Strain PerGlxl, was enriched and isolated from marine sediment samples with glyoxylate as sole carbon and energy source. The guanineplus-cytosine content of the DNA was 44.1 +/-0.2 mol %. Glyoxylate was utilized as the only substrate and was stoichiometrically degraded to carbon dioxide, hydrogen, and glycolate. An acetyl-CoA and ADP-dependent glyoxylate converting enzyme activity, malic enzyme, and pyruvate synthase were found at activities sufficient for growth (>/= 0.25 U x mg protein- 1). These findings allow to design a new degradation pathway for glyoxylate: glyoxylate is condensed with acetyl-CoA to form malyl-CoA; the free energy of the thioester linkage in malyl-CoA is conserved by substrate level phosphorylation. Part of the electrons released during glyoxylate oxidation to CO2 reduce a small fraction of glyoxylate to glycolate.

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570 Biowissenschaften, Biologie
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Glyoxylate, Anaerobic degradation, Malic enzyme, Substrate level phosphorylation
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ISO 690FRIEDRICH, Michael, Bernhard SCHINK, 1991. Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium. In: Archives of Microbiology. 1991, 156(5), pp. 392-397. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248716
BibTex
@article{Friedrich1991Ferme-7523,
  year={1991},
  doi={10.1007/BF00248716},
  title={Fermentative degradation of glyoxylate by a new strictly anaerobic bacterium},
  number={5},
  volume={156},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={392--397},
  author={Friedrich, Michael and Schink, Bernhard}
}
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