Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate

Müller, Jochen A.; Galushko, Alexander S.; Kappler, Andreas; Schink, Bernhard

Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate

Dateien

Anaerobic_degradation_of_m_cresol_by_1999.pdfGröße: 211.61 KBDownloads: 472

Datum

1999

Autor:innen

Müller, Jochen A.

Galushko, Alexander S.

Kappler, Andreas

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1999, 172(5), pp. 287-294. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050782

Zusammenfassung

The anaerobic bacterium Desulfobacterium cetonicum oxidized m-cresol completely with sulfate as electron acceptor. During growth, 3-hydroxybenzylsuccinate (identified by gas chromatography/mass spectroscopy and by comparison of high-performance liquid chromatography retention time and UV spectrum with a chemically synthesized reference compound) accumulated in the medium. This finding indicates that the methyl group of mcresol is activated by addition to fumarate as in the case of anaerobic toluene metabolism. In cell-free extracts of D. cetonicum, the formation of 3-hydroxybenzylsuccinate from m-cresol and fumarate was detected at an activity of 0.5 nmol min 1 (mg protein) 1. This reaction depended strictly on anoxic assay conditions. Treatment with air resulted in a complete loss of activity; however, some activity could be recovered after restoring anoxic conditions. The activity was slightly membrane-associated. 3-Hydroxybenzylsuccinate was degraded via CoA thioesterification and further oxidation to 3-hydroxybenzoyl-CoA as subsequent steps in the degradation pathway.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Anaerobic degradation, Aromaticcompounds, m-Cresol, Toluene, 3-Hydroxybenzylsuccinate, Sulfate-reducing bacteria, Desulfobacterium cetonicum

Zitieren

ISO 690

MÜLLER, Jochen A., Alexander S. GALUSHKO, Andreas KAPPLER, Bernhard SCHINK, 1999. Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate. In: Archives of Microbiology. 1999, 172(5), pp. 287-294. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050782

BibTex

@article{Muller1999Anaer-8273,
  year={1999},
  doi={10.1007/s002030050782},
  title={Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate},
  number={5},
  volume={172},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={287--294},
  author={Müller, Jochen A. and Galushko, Alexander S. and Kappler, Andreas and Schink, Bernhard}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8273">
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8273/1/Anaerobic_degradation_of_m_cresol_by_1999.pdf"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">The anaerobic bacterium Desulfobacterium cetonicum oxidized m-cresol completely with sulfate as electron acceptor. During growth, 3-hydroxybenzylsuccinate (identified by gas chromatography/mass spectroscopy and by comparison of high-performance liquid chromatography retention time and UV spectrum with a chemically synthesized reference compound) accumulated in the medium. This finding indicates that the methyl group of mcresol is activated by addition to fumarate as in the case of anaerobic toluene metabolism. In cell-free extracts of D. cetonicum, the formation of 3-hydroxybenzylsuccinate from m-cresol and fumarate was detected at an activity of 0.5 nmol min 1 (mg protein) 1. This reaction depended strictly on anoxic assay conditions. Treatment with air resulted in a complete loss of activity; however, some activity could be recovered after restoring anoxic conditions. The activity was slightly membrane-associated. 3-Hydroxybenzylsuccinate was degraded via CoA thioesterification and further oxidation to 3-hydroxybenzoyl-CoA as subsequent steps in the degradation pathway.</dcterms:abstract>
    <dcterms:title>Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate</dcterms:title>
    <dc:creator>Galushko, Alexander S.</dc:creator>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 172 (1999), 5, pp. 287-294</dcterms:bibliographicCitation>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8273/1/Anaerobic_degradation_of_m_cresol_by_1999.pdf"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Müller, Jochen A.</dc:contributor>
    <dc:creator>Kappler, Andreas</dc:creator>
    <dc:creator>Müller, Jochen A.</dc:creator>
    <dc:contributor>Galushko, Alexander S.</dc:contributor>
    <dc:contributor>Kappler, Andreas</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:19Z</dcterms:available>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:issued>1999</dcterms:issued>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:19Z</dc:date>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8273"/>
    <dc:format>application/pdf</dc:format>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein