@article{Muller1999Anaer-8273,
  year={1999},
  doi={10.1007/s002030050782},
  title={Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate},
  number={5},
  volume={172},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={287--294},
  author={Müller, Jochen A. and Galushko, Alexander S. and Kappler, Andreas and Schink, Bernhard}
}

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    <dcterms:abstract xml:lang="eng">The anaerobic bacterium Desulfobacterium cetonicum oxidized m-cresol completely with sulfate as electron acceptor. During growth, 3-hydroxybenzylsuccinate (identified by gas chromatography/mass spectroscopy and by comparison of high-performance liquid chromatography retention time and UV spectrum with a chemically synthesized reference compound) accumulated in the medium. This finding indicates that the methyl group of mcresol is activated by addition to fumarate as in the case of anaerobic toluene metabolism. In cell-free extracts of D. cetonicum, the formation of 3-hydroxybenzylsuccinate from m-cresol and fumarate was detected at an activity of 0.5 nmol min 1 (mg protein) 1. This reaction depended strictly on anoxic assay conditions. Treatment with air resulted in a complete loss of activity; however, some activity could be recovered after restoring anoxic conditions. The activity was slightly membrane-associated. 3-Hydroxybenzylsuccinate was degraded via CoA thioesterification and further oxidation to 3-hydroxybenzoyl-CoA as subsequent steps in the degradation pathway.</dcterms:abstract>
    <dcterms:title>Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate</dcterms:title>
    <dc:creator>Galushko, Alexander S.</dc:creator>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 172 (1999), 5, pp. 287-294</dcterms:bibliographicCitation>
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    <dc:contributor>Müller, Jochen A.</dc:contributor>
    <dc:creator>Kappler, Andreas</dc:creator>
    <dc:creator>Müller, Jochen A.</dc:creator>
    <dc:contributor>Galushko, Alexander S.</dc:contributor>
    <dc:contributor>Kappler, Andreas</dc:contributor>
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    <dc:contributor>Schink, Bernhard</dc:contributor>
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    <dcterms:issued>1999</dcterms:issued>
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