Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal

Gachon, Claire M. M.; Strittmatter, Martina; Müller, Dieter G.; Kleinteich, Julia; Küpper, Frithjof C.

Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2009

Autor:innen

Gachon, Claire M. M.

Strittmatter, Martina

Müller, Dieter G.

Kleinteich, Julia

Küpper, Frithjof C.

DOI (zitierfähiger Link)

10.1128/AEM.01885-08

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Applied and Environmental Microbiology ; 75 (2009), 2. - S. 322-328. - ISSN 0099-2240. - eISSN 1098-5336

Zusammenfassung

In the marine environment, a growing body of evidence points to parasites as key players in the control of population dynamics and overall ecosystem structure. However, their prevalence and impact on marine macroalgal communities remain virtually unknown. Indeed, infectious diseases of seaweeds are largely underdocumented, partly because of the expertise required to diagnose them with a microscope. Over the last few years, however, real-time quantitative PCR (qPCR) has emerged as a rapid and reliable alternative to visual symptom scoring for monitoring pathogens. Thus, we present here a qPCR assay suitable for the detection and quantification of the intracellular oomycete pathogen Eurychasma dicksonii in its ectocarpalean and laminarialean brown algal hosts. qPCR and microscopic observations made of laboratory-controlled cultures revealed that clonal brown algal strains exhibit different levels of resistance against Eurychasma, ranging from high susceptibility to complete absence of symptoms. This observation strongly argues for the existence of a genetic determinism for disease resistance in brown algae, which would have broad implications for the dynamics and genetic structure of natural populations. We also used qPCR for the rapid detection of Eurychasma in filamentous brown algae collected in Northern Europe and South America and found that the assay is specific, robust, and widely applicable to field samples. Hence, this study opens the perspective of combining large-scale disease monitoring in the field with laboratory-controlled experiments on the genome model seaweed Ectocarpus siliculosus to improve our understanding of brown algal diseases.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

GACHON, Claire M. M., Martina STRITTMATTER, Dieter G. MÜLLER, Julia KLEINTEICH, Frithjof C. KÜPPER, 2009. Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal. In: Applied and Environmental Microbiology. 75(2), pp. 322-328. ISSN 0099-2240. eISSN 1098-5336. Available under: doi: 10.1128/AEM.01885-08

BibTex

@article{Gachon2009Detec-32173,
  year={2009},
  doi={10.1128/AEM.01885-08},
  title={Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal},
  number={2},
  volume={75},
  issn={0099-2240},
  journal={Applied and Environmental Microbiology},
  pages={322--328},
  author={Gachon, Claire M. M. and Strittmatter, Martina and Müller, Dieter G. and Kleinteich, Julia and Küpper, Frithjof C.}
}

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Universitätsbibliographie

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