@article{GarciaSeyda2023-08Naive-67740,
  year={2023},
  doi={10.1016/j.isci.2023.107695},
  title={Naïve T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P},
  journal={iScience},
  author={Garcia-Seyda, Nicolas and Song, Solene and Seveau de Noray, Valentine and David-Broglio, Luc and Matti, Christoph and Artinger, Marc and Dupuy, Florian and Biarnes-Pelicot, Martine and Legler, Daniel F. and Theodoly, Olivier}
}

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    <dc:contributor>Artinger, Marc</dc:contributor>
    <dc:contributor>Garcia-Seyda, Nicolas</dc:contributor>
    <dc:creator>Seveau de Noray, Valentine</dc:creator>
    <dc:creator>Song, Solene</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Dupuy, Florian</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/67740"/>
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    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:language>eng</dc:language>
    <dcterms:issued>2023-08</dcterms:issued>
    <dc:creator>Matti, Christoph</dc:creator>
    <dc:creator>David-Broglio, Luc</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-09-06T14:09:42Z</dcterms:available>
    <dc:creator>Dupuy, Florian</dc:creator>
    <dc:creator>Legler, Daniel F.</dc:creator>
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    <dcterms:abstract>Naïve T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naïve T lymphocyte migration along defined gradients is missing. Here, we used a reductionist approach to study the real-time single-cell response of naïve T lymphocytes to CCL21 and serum rich in bioactive S1P. Using microfluidic and micropatterning ad hoc tools, we show that CCL21 triggers stable polarization and long-range chemotaxis of cells, whereas S1P-rich serum triggers a transient polarization only and no significant displacement, potentially representing a brief transmigration step through exit portals. Our in vitro data thus suggest that naïve T lymphocyte chemotax long distances to CCL21 but not towards a source of bioactive S1P.</dcterms:abstract>
    <dc:contributor>Matti, Christoph</dc:contributor>
    <dc:contributor>Theodoly, Olivier</dc:contributor>
    <dc:creator>Artinger, Marc</dc:creator>
    <dcterms:title>Naïve T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P</dcterms:title>
    <dc:contributor>Song, Solene</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-09-06T14:09:42Z</dc:date>
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    <dc:contributor>Seveau de Noray, Valentine</dc:contributor>
    <dc:contributor>David-Broglio, Luc</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:creator>Theodoly, Olivier</dc:creator>
    <dc:contributor>Biarnes-Pelicot, Martine</dc:contributor>
    <dc:creator>Garcia-Seyda, Nicolas</dc:creator>
    <dc:contributor>Legler, Daniel F.</dc:contributor>
    <dc:creator>Biarnes-Pelicot, Martine</dc:creator>
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