Naïve T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P

Lade...
Vorschaubild
Dateien
Garcia-Seyda_2-blvnooh26i055.pdf
Garcia-Seyda_2-blvnooh26i055.pdfGröße: 5.29 MBDownloads: 11
Datum
2023
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
European Union (EU): 713750
Projekt
Open Access-Veröffentlichung
Open Access Gold
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
iScience. Elsevier. 2023, 26(10), 107695. ISSN 2589-0042. eISSN 2589-0042. Available under: doi: 10.1016/j.isci.2023.107695
Zusammenfassung

Naïve T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naïve T lymphocyte migration along defined gradients is missing. Here, we used a reductionist approach to study the real-time single-cell response of naïve T lymphocytes to CCL21 and serum rich in bioactive S1P. Using microfluidic and micropatterning ad hoc tools, we show that CCL21 triggers stable polarization and long-range chemotaxis of cells, whereas S1P-rich serum triggers a transient polarization only and no significant displacement, potentially representing a brief transmigration step through exit portals. Our in vitro data thus suggest that naïve T lymphocyte chemotax long distances to CCL21 but not towards a source of bioactive S1P.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Multidisciplinary
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690GARCIA-SEYDA, Nicolas, Solene SONG, Valentine SEVEAU DE NORAY, Luc DAVID-BROGLIO, Christoph MATTI, Marc ARTINGER, Florian DUPUY, Martine BIARNES-PELICOT, Daniel F. LEGLER, Olivier THEODOLY, 2023. Naïve T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P. In: iScience. Elsevier. 2023, 26(10), 107695. ISSN 2589-0042. eISSN 2589-0042. Available under: doi: 10.1016/j.isci.2023.107695
BibTex
@article{GarciaSeyda2023-10Naive-67740,
  year={2023},
  doi={10.1016/j.isci.2023.107695},
  title={Naïve T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P},
  number={10},
  volume={26},
  issn={2589-0042},
  journal={iScience},
  author={Garcia-Seyda, Nicolas and Song, Solene and Seveau de Noray, Valentine and David-Broglio, Luc and Matti, Christoph and Artinger, Marc and Dupuy, Florian and Biarnes-Pelicot, Martine and Legler, Daniel F. and Theodoly, Olivier},
  note={Article Number: 107695}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/67740">
    <dc:contributor>Artinger, Marc</dc:contributor>
    <dc:contributor>Garcia-Seyda, Nicolas</dc:contributor>
    <dc:creator>Seveau de Noray, Valentine</dc:creator>
    <dc:creator>Song, Solene</dc:creator>
    <dcterms:issued>2023-10</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Dupuy, Florian</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/67740"/>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Matti, Christoph</dc:creator>
    <dc:creator>David-Broglio, Luc</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-09-06T14:09:42Z</dcterms:available>
    <dc:creator>Dupuy, Florian</dc:creator>
    <dc:creator>Legler, Daniel F.</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract>Naïve T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics relies on sphingolipid S1P sensing by S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naïve T lymphocyte migration along defined gradients is missing. Here, we used a reductionist approach to study the real-time single-cell response of naïve T lymphocytes to CCL21 and serum rich in bioactive S1P. Using microfluidic and micropatterning ad hoc tools, we show that CCL21 triggers stable polarization and long-range chemotaxis of cells, whereas S1P-rich serum triggers a transient polarization only and no significant displacement, potentially representing a brief transmigration step through exit portals. Our in vitro data thus suggest that naïve T lymphocyte chemotax long distances to CCL21 but not towards a source of bioactive S1P.</dcterms:abstract>
    <dc:contributor>Matti, Christoph</dc:contributor>
    <dc:contributor>Theodoly, Olivier</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/67740/1/Garcia-Seyda_2-blvnooh26i055.pdf"/>
    <dc:creator>Artinger, Marc</dc:creator>
    <dcterms:title>Naïve T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P</dcterms:title>
    <dc:contributor>Song, Solene</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2023-09-06T14:09:42Z</dc:date>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Seveau de Noray, Valentine</dc:contributor>
    <dc:contributor>David-Broglio, Luc</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:creator>Theodoly, Olivier</dc:creator>
    <dc:contributor>Biarnes-Pelicot, Martine</dc:contributor>
    <dc:creator>Garcia-Seyda, Nicolas</dc:creator>
    <dc:contributor>Legler, Daniel F.</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/67740/1/Garcia-Seyda_2-blvnooh26i055.pdf"/>
    <dc:creator>Biarnes-Pelicot, Martine</dc:creator>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen