Novel perspectives for the application of total internal reflection microscopy

Volpe, Giovanni; Brettschneider, Thomas; Helden, Laurent; Bechinger, Clemens

Novel perspectives for the application of total internal reflection microscopy

Files

Volpe_0-413921.pdf(729.12 KB)

Date

2009

Authors

Volpe, Giovanni

Brettschneider, Thomas

Helden, Laurent

Bechinger, Clemens

Publication type

Journal article

Publication status

Published

Published in

Optics Express ; 17 (2009), 26. - pp. 23975-23985. - eISSN 1094-4087

Abstract

Total Internal Reflection Microscopy (TIRM) is a sensitive non-invasive technique to measure the interaction potentials between a colloidal particle and a wall with femtonewton resolution. The equilibrium distribution of the particle-wall separation distance z is sampled monitoring the intensity I scattered by the Brownian particle under evanescent illumination. Central to the data analysis is the knowledge of the relation between I and the corresponding z, which typically must be known a priori. This poses considerable constraints to the experimental conditions where TIRM can be applied (short penetration depth of the evanescent wave, transparent surfaces). Here, we introduce a method to experimentally determine I(z) by relying only on the distance-dependent particle-wall hydrodynamic interactions. We demonstrate that this method largely extends the range of conditions accessible with TIRM, and even allows measurements on highly reflecting gold surfaces where multiple reflections lead to a complex (z).

Subject (DDC)

530 Physics

Cite This

ISO 690

VOLPE, Giovanni, Thomas BRETTSCHNEIDER, Laurent HELDEN, Clemens BECHINGER, 2009. Novel perspectives for the application of total internal reflection microscopy. In: Optics Express. 17(26), pp. 23975-23985. eISSN 1094-4087

BibTex

@article{Volpe2009-12-21Novel-39466,
  year={2009},
  title={Novel perspectives for the application of total internal reflection microscopy},
  number={26},
  volume={17},
  journal={Optics Express},
  pages={23975--23985},
  author={Volpe, Giovanni and Brettschneider, Thomas and Helden, Laurent and Bechinger, Clemens}
}

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