Dynamics of Snake-like Swarming Behavior of Vibrio alginolyticus

Böttcher, Thomas; Elliott, Hunter L.; Clardy, Jon

Dynamics of Snake-like Swarming Behavior of Vibrio alginolyticus

Files

There are no files associated with this item.

Date

2016

Authors

Böttcher, Thomas

Elliott, Hunter L.

Clardy, Jon

DOI (citable link)

10.1016/j.bpj.2015.12.037

Collections

Chemie
Zukunftskolleg

Publication type

Journal article

Publication status

Published

Published in

Biophysical Journal ; 110 (2016), 4. - pp. 981-992. - ISSN 0006-3495. - eISSN 1542-0086

Abstract

Swarming represents a special case of bacterial behavior where motile bacteria migrate rapidly and collectively on surfaces. Swarming and swimming motility of bacteria has been studied well for rigid, self-propelled rods. In this study we report a strain of Vibrio alginolyticus, a species that exhibits similar collective motility but a fundamentally different cell morphology with highly flexible snake-like swarming cells. Investigating swarming dynamics requires high-resolution imaging of single cells with coverage over a large area: thousands of square microns. Researchers previously have employed various methods of motion analysis but largely for rod-like bacteria. We employ temporal variance analysis of a short time-lapse microscopic image series to capture the motion dynamics of swarming Vibrio alginolyticus at cellular resolution over hundreds of microns. Temporal variance is a simple and broadly applicable method for analyzing bacterial swarming behavior in two and three dimensions with both high-resolution and wide-spatial coverage. This study provides detailed insights into the swarming architecture and dynamics of Vibrio alginolyticus isolate B522 on carrageenan agar that may lay the foundation for swarming studies of snake-like, nonrod-shaped motile cell types.

Subject (DDC)

540 Chemistry

Cite This

ISO 690

BÖTTCHER, Thomas, Hunter L. ELLIOTT, Jon CLARDY, 2016. Dynamics of Snake-like Swarming Behavior of Vibrio alginolyticus. In: Biophysical Journal. 110(4), pp. 981-992. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2015.12.037

BibTex

@article{Bottcher2016-02Dynam-33664,
  year={2016},
  doi={10.1016/j.bpj.2015.12.037},
  title={Dynamics of Snake-like Swarming Behavior of Vibrio alginolyticus},
  number={4},
  volume={110},
  issn={0006-3495},
  journal={Biophysical Journal},
  pages={981--992},
  author={Böttcher, Thomas and Elliott, Hunter L. and Clardy, Jon}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/33664">
    <dc:creator>Elliott, Hunter L.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:contributor>Elliott, Hunter L.</dc:contributor>
    <dc:creator>Böttcher, Thomas</dc:creator>
    <dc:creator>Clardy, Jon</dc:creator>
    <dcterms:abstract xml:lang="eng">Swarming represents a special case of bacterial behavior where motile bacteria migrate rapidly and collectively on surfaces. Swarming and swimming motility of bacteria has been studied well for rigid, self-propelled rods. In this study we report a strain of Vibrio alginolyticus, a species that exhibits similar collective motility but a fundamentally different cell morphology with highly flexible snake-like swarming cells. Investigating swarming dynamics requires high-resolution imaging of single cells with coverage over a large area: thousands of square microns. Researchers previously have employed various methods of motion analysis but largely for rod-like bacteria. We employ temporal variance analysis of a short time-lapse microscopic image series to capture the motion dynamics of swarming Vibrio alginolyticus at cellular resolution over hundreds of microns. Temporal variance is a simple and broadly applicable method for analyzing bacterial swarming behavior in two and three dimensions with both high-resolution and wide-spatial coverage. This study provides detailed insights into the swarming architecture and dynamics of Vibrio alginolyticus isolate B522 on carrageenan agar that may lay the foundation for swarming studies of snake-like, nonrod-shaped motile cell types.</dcterms:abstract>
    <dc:contributor>Clardy, Jon</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:language>eng</dc:language>
    <dc:contributor>Böttcher, Thomas</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33664"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-04-22T11:42:30Z</dc:date>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-04-22T11:42:30Z</dcterms:available>
    <dcterms:title>Dynamics of Snake-like Swarming Behavior of Vibrio alginolyticus</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:issued>2016-02</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
  </rdf:Description>
</rdf:RDF>

Bibliography of Konstanz

Yes