Different Enzymatic Processing of γ-Phosphoramidate and γ-Phosphoester-Modified ATP Analogues

Ermert, Susanne; Hacker, Stephan M.; Buntru, Alexander; Scheffner, Martin; Hauck, Christof R.; Marx, Andreas

Different Enzymatic Processing of γ-Phosphoramidate and γ-Phosphoester-Modified ATP Analogues

Dateien

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Datum

2017

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

ChemBioChem. 2017, 18(4), pp. 378-381. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600590

Zusammenfassung

Monitoring the activity of ATP-consuming enzymes provides the basis for elucidating their modes of action and regulation. Although a number of ATP analogues have been developed for this, their scope is restricted because of the limited acceptance by respective enzymes. In order to clarify which kind of phosphate-modified ATP analogues are accepted by the α-β-phosphoanhydride-cleaving ubiquitin-activating enzyme 1 (UBA1) and the β-γ-phosphoanhydride-cleaving focal adhesion kinase (FAK), we tested phosphoramidate- and phosphoester-modified ATP analogues. UBA1 and FAK were able to convert phosphoramidate-modified ATP analogues, even with a bulky modification like biotin. In contrast, a phosphoester-modified analogue was poorly accepted. These results demonstrate that minor variations in the design of ATP analogues for monitoring ATP utilization have a significant impact on enzymatic acceptance.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

ERMERT, Susanne, Stephan M. HACKER, Alexander BUNTRU, Martin SCHEFFNER, Christof R. HAUCK, Andreas MARX, 2017. Different Enzymatic Processing of γ-Phosphoramidate and γ-Phosphoester-Modified ATP Analogues. In: ChemBioChem. 2017, 18(4), pp. 378-381. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600590

BibTex

@article{Ermert2017-02-16Diffe-38686,
  year={2017},
  doi={10.1002/cbic.201600590},
  title={Different Enzymatic Processing of γ-Phosphoramidate and γ-Phosphoester-Modified ATP Analogues},
  number={4},
  volume={18},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={378--381},
  author={Ermert, Susanne and Hacker, Stephan M. and Buntru, Alexander and Scheffner, Martin and Hauck, Christof R. and Marx, Andreas}
}

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