Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3

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2015
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Kalinowska, Kamila
Goodman, Kaija
Cuyas, Laura
Anzenberger, Franziska
Alkofer, Angela
Paz-Ares, Javier
Braun, Pascal
Rubio, Vicente
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Ubiquitination is a signal for various cellular processes, including for endocytic degradation of plasma membrane cargos. Ubiquitinating as well as deubiquitinating enzymes (DUBs) can regulate these processes by modifying the ubiquitination status of target protein. Although accumulating evidence points to the important regulatory role of DUBs, the molecular basis of their regulation is still not well understood. Associated molecule with the SH3 domain of signal transduction adaptor molecule (STAM) (AMSH) is a conserved metalloprotease DUB in eukaryotes. AMSH proteins interact with components of the endosomal sorting complex required for transport (ESCRT) and are implicated in intracellular trafficking. To investigate how the function of AMSH is regulated at the cellular level, we carried out an interaction screen for the Arabidopsis AMSH proteins and identified the Arabidopsis homolog of apoptosis-linked gene-2 interacting protein X (ALIX) as a protein interacting with AMSH3 in vitro and in vivo. Analysis of alix knockout mutants in Arabidopsis showed that ALIX is essential for plant growth and development and that ALIX is important for the biogenesis of the vacuole and multivesicular bodies (MVBs). Cell biological analysis revealed that ALIX and AMSH3 colocalize on late endosomes. Although ALIX did not stimulate AMSH3 activity in vitro, in the absence of ALIX, AMSH3 localization on endosomes was abolished. Taken together, our data indicate that ALIX could function as an important regulator for AMSH3 function at the late endosomes.

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570 Biowissenschaften, Biologie
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Arabidopsis, intracellular trafficking, ubiquitin, ESCRT-III
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ISO 690KALINOWSKA, Kamila, Marie-Kristin NAGEL, Kaija GOODMAN, Laura CUYAS, Franziska ANZENBERGER, Angela ALKOFER, Javier PAZ-ARES, Pascal BRAUN, Vicente RUBIO, Erika ISONO, 2015. Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3. In: Proceedings of the National Academy of Sciences of the United States of America (PNAS). 2015, 112(40), pp. E5543-E5551. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.1510516112
BibTex
@article{Kalinowska2015-10-06Arabi-38077,
  year={2015},
  doi={10.1073/pnas.1510516112},
  title={Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3},
  number={40},
  volume={112},
  issn={0027-8424},
  journal={Proceedings of the National Academy of Sciences of the United States of America (PNAS)},
  pages={E5543--E5551},
  author={Kalinowska, Kamila and Nagel, Marie-Kristin and Goodman, Kaija and Cuyas, Laura and Anzenberger, Franziska and Alkofer, Angela and Paz-Ares, Javier and Braun, Pascal and Rubio, Vicente and Isono, Erika}
}
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