Determination of vitellogenin kinetics in male fathead minnows (Pimephales promelas)

Schmid, Tobias; Gonzales-Valero, Juan; Rufli, Hans; Dietrich, Daniel R.

Determination of vitellogenin kinetics in male fathead minnows (Pimephales promelas)

Dateien

Determination_of_vitellogenin_kinetics_in_male_fathead_minnows.pdf(156.71 KB)

Datum

2002

Autor:innen

Schmid, Tobias

Gonzales-Valero, Juan

Rufli, Hans

Dietrich, Daniel R.

Publikationstyp

Zeitschriftenartikel

Erschienen in

Toxicology letters ; 131 (2002), 1-2. - S. 65-74. - ISSN 0378-4274

Zusammenfassung

A lack of knowledge persists concerning the combination of kinetics on protein and mRNA levels of the most commonly used biomarker for estrogenic influences vitellogenin (VTG). Consequently, male fathead minnows were exposed to 17α-ethinylestradiol (EE2) for 35 days, followed by an equally long depuration period in a flow-through system. VTG mRNA levels reached a plateau after 3 days of exposure, which remained stable until 3 days after EE2 removal. Control levels were re-attained within 7 days of the depuration phase. VTG protein accumulated in the plasma following a two-phased model. The first phase depicting an exponential increase lasted 15 days and was followed by a saturation phase approaching a plateau of approximately 47 mg VTG/ml plasma. Clearance kinetics could be described by a two-compartment open model, with half-lives of 2.17 and 21.32 days for the α- and β-phases, respectively. In addition, a high VTG protein synthesis rate seemed to adversely affect fitness and mortality of the fish.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Vitellogenin,Half-life,Kinetics,Fathead minnow,Ethinylestradiol,Fitness

Zitieren

ISO 690

SCHMID, Tobias, Juan GONZALES-VALERO, Hans RUFLI, Daniel R. DIETRICH, 2002. Determination of vitellogenin kinetics in male fathead minnows (Pimephales promelas). In: Toxicology letters. 131(1-2), pp. 65-74. ISSN 0378-4274. Available under: doi: 10.1016/S0378-4274(02)00043-7

BibTex

@article{Schmid2002Deter-7744,
  year={2002},
  doi={10.1016/S0378-4274(02)00043-7},
  title={Determination of vitellogenin kinetics in male fathead minnows (Pimephales promelas)},
  number={1-2},
  volume={131},
  issn={0378-4274},
  journal={Toxicology letters},
  pages={65--74},
  author={Schmid, Tobias and Gonzales-Valero, Juan and Rufli, Hans and Dietrich, Daniel R.}
}

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