Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair
Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair
Lade...
Datum
2020
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
eISSN
item.preview.dc.identifier.isbn
Bibliografische Daten
Verlag
Schriftenreihe
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
EU-Projektnummer
Projekt
Open Access-Veröffentlichung
Sammlungen
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
International Journal of Molecular Sciences ; 21 (2020), 5. - 1638. - MDPI. - ISSN 1661-6596. - eISSN 1422-0067
Zusammenfassung
The endogenous gasotransmitter H2S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H2S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H2S-releasing agents on the growth of stem cells have not been fully investigated. H2S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H2S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H2S agents on the cell growth and differentiation of cardiac Lin- Sca1+ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH-garlic conjugates (GSGa) on cMSC compared to other H2S-releasing agents, such as Na2S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell-cell adhesion and commitment to differentiation. These results highlight the effects of H2S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
hydrogen sulfide; garlic; regenerative medicine; oxidative stress; MSCs; organosulfur compounds; cell migration; cell differentiation
Konferenz
Rezension
undefined / . - undefined, undefined. - (undefined; undefined)
Zitieren
ISO 690
DI GIOVANNI, Emilia, Silvia BUONVINO, Ivano AMELIO, Sonia MELINO, 2020. Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair. In: International Journal of Molecular Sciences. MDPI. 21(5), 1638. ISSN 1661-6596. eISSN 1422-0067. Available under: doi: 10.3390/ijms21051638BibTex
@article{DiGiovanni2020-02-28Gluta-56831, year={2020}, doi={10.3390/ijms21051638}, title={Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair}, number={5}, volume={21}, issn={1661-6596}, journal={International Journal of Molecular Sciences}, author={Di Giovanni, Emilia and Buonvino, Silvia and Amelio, Ivano and Melino, Sonia}, note={Article Number: 1638} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/56831"> <dc:creator>Di Giovanni, Emilia</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56831/3/DiGiovanni_2-ajq9xzc68yah7.pdf"/> <dcterms:title>Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair</dcterms:title> <dcterms:abstract xml:lang="eng">The endogenous gasotransmitter H<sub>2</sub>S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H<sub>2</sub>S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H<sub>2</sub>S-releasing agents on the growth of stem cells have not been fully investigated. H<sub>2</sub>S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H<sub>2</sub>S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H<sub>2</sub>S agents on the cell growth and differentiation of cardiac Lin<sup>-</sup> Sca1<sup>+</sup> human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH-garlic conjugates (GSGa) on cMSC compared to other H<sub>2</sub>S-releasing agents, such as Na<sub>2</sub>S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell-cell adhesion and commitment to differentiation. These results highlight the effects of H<sub>2</sub>S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.</dcterms:abstract> <dc:creator>Buonvino, Silvia</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Buonvino, Silvia</dc:contributor> <dc:contributor>Amelio, Ivano</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:issued>2020-02-28</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-11T10:17:52Z</dcterms:available> <dc:creator>Amelio, Ivano</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56831/3/DiGiovanni_2-ajq9xzc68yah7.pdf"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/56831"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:language>eng</dc:language> <dc:rights>Attribution 4.0 International</dc:rights> <dc:contributor>Di Giovanni, Emilia</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Melino, Sonia</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-11T10:17:52Z</dc:date> <dc:contributor>Melino, Sonia</dc:contributor> </rdf:Description> </rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja