Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair

Di Giovanni, Emilia; Buonvino, Silvia; Amelio, Ivano; Melino, Sonia

Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair

Dateien

DiGiovanni_2-ajq9xzc68yah7.pdfGröße: 8.61 MBDownloads: 86

Datum

2020

Autor:innen

Di Giovanni, Emilia

Buonvino, Silvia

Amelio, Ivano

Melino, Sonia

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

International Journal of Molecular Sciences. MDPI. 2020, 21(5), 1638. ISSN 1661-6596. eISSN 1422-0067. Available under: doi: 10.3390/ijms21051638

Zusammenfassung

The endogenous gasotransmitter H₂S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H₂S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H₂S-releasing agents on the growth of stem cells have not been fully investigated. H₂S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H₂S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H₂S agents on the cell growth and differentiation of cardiac Lin^- Sca1⁺ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH-garlic conjugates (GSGa) on cMSC compared to other H₂S-releasing agents, such as Na₂S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell-cell adhesion and commitment to differentiation. These results highlight the effects of H₂S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

hydrogen sulfide; garlic; regenerative medicine; oxidative stress; MSCs; organosulfur compounds; cell migration; cell differentiation

Zitieren

ISO 690

DI GIOVANNI, Emilia, Silvia BUONVINO, Ivano AMELIO, Sonia MELINO, 2020. Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair. In: International Journal of Molecular Sciences. MDPI. 2020, 21(5), 1638. ISSN 1661-6596. eISSN 1422-0067. Available under: doi: 10.3390/ijms21051638

BibTex

@article{DiGiovanni2020-02-28Gluta-56831,
  year={2020},
  doi={10.3390/ijms21051638},
  title={Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair},
  number={5},
  volume={21},
  issn={1661-6596},
  journal={International Journal of Molecular Sciences},
  author={Di Giovanni, Emilia and Buonvino, Silvia and Amelio, Ivano and Melino, Sonia},
  note={Article Number: 1638}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/56831">
    <dc:creator>Di Giovanni, Emilia</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56831/3/DiGiovanni_2-ajq9xzc68yah7.pdf"/>
    <dcterms:title>Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair</dcterms:title>
    <dcterms:abstract xml:lang="eng">The endogenous gasotransmitter H&lt;sub&gt;2&lt;/sub&gt;S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H&lt;sub&gt;2&lt;/sub&gt;S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H&lt;sub&gt;2&lt;/sub&gt;S-releasing agents on the growth of stem cells have not been fully investigated. H&lt;sub&gt;2&lt;/sub&gt;S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H&lt;sub&gt;2&lt;/sub&gt;S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H&lt;sub&gt;2&lt;/sub&gt;S agents on the cell growth and differentiation of cardiac Lin&lt;sup&gt;-&lt;/sup&gt; Sca1&lt;sup&gt;+&lt;/sup&gt; human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH-garlic conjugates (GSGa) on cMSC compared to other H&lt;sub&gt;2&lt;/sub&gt;S-releasing agents, such as Na&lt;sub&gt;2&lt;/sub&gt;S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell-cell adhesion and commitment to differentiation. These results highlight the effects of H&lt;sub&gt;2&lt;/sub&gt;S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.</dcterms:abstract>
    <dc:creator>Buonvino, Silvia</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Buonvino, Silvia</dc:contributor>
    <dc:contributor>Amelio, Ivano</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:issued>2020-02-28</dcterms:issued>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-11T10:17:52Z</dcterms:available>
    <dc:creator>Amelio, Ivano</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56831/3/DiGiovanni_2-ajq9xzc68yah7.pdf"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/56831"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:contributor>Di Giovanni, Emilia</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Melino, Sonia</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-11T10:17:52Z</dc:date>
    <dc:contributor>Melino, Sonia</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein

Begutachtet

Ja