Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair

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2020
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Di Giovanni, Emilia
Buonvino, Silvia
Melino, Sonia
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The endogenous gasotransmitter H2S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H2S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H2S-releasing agents on the growth of stem cells have not been fully investigated. H2S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H2S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H2S agents on the cell growth and differentiation of cardiac Lin- Sca1+ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH-garlic conjugates (GSGa) on cMSC compared to other H2S-releasing agents, such as Na2S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell-cell adhesion and commitment to differentiation. These results highlight the effects of H2S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.

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570 Biowissenschaften, Biologie
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hydrogen sulfide; garlic; regenerative medicine; oxidative stress; MSCs; organosulfur compounds; cell migration; cell differentiation
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ISO 690DI GIOVANNI, Emilia, Silvia BUONVINO, Ivano AMELIO, Sonia MELINO, 2020. Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair. In: International Journal of Molecular Sciences. MDPI. 2020, 21(5), 1638. ISSN 1661-6596. eISSN 1422-0067. Available under: doi: 10.3390/ijms21051638
BibTex
@article{DiGiovanni2020-02-28Gluta-56831,
  year={2020},
  doi={10.3390/ijms21051638},
  title={Glutathione-Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair},
  number={5},
  volume={21},
  issn={1661-6596},
  journal={International Journal of Molecular Sciences},
  author={Di Giovanni, Emilia and Buonvino, Silvia and Amelio, Ivano and Melino, Sonia},
  note={Article Number: 1638}
}
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