Publikation: Force-induced structural transitions in cross-linked DNA films
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2008
Autor:innen
André, Alexander
Fontaine-Vive, Fabien
Fischer, Theo
Forsyth, V. Trevor
Herausgeber:innen
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European Biophysics Journal. 2008, 37(6), pp. 749-757. ISSN 0175-7571. Available under: doi: 10.1007/s00249-008-0257-4
Zusammenfassung
We report on the preparation and characterization of wet-spun films of sodium DNA in which intermolecular cross-links were introduced following formaldehyde treatment. Raman scattering shows that the DNA in moderately cross-linked films is mainly in the B conformation. Stretching experiments show a transition from plastic to elastomeric behavior with increasing exposure to the cross-linking agent. Elastomeric DNA films are strongly disordered. X-ray diffraction shows that stretching of moderately cross-linked films under controlled high humidity conditions results in increased molecular orientation as well as the appearance of meridional reflections at 7.4–7.8 and 8.2 Å. These reflections are not observed for any of the classical conformations associated with mixed sequence DNA, and may arise from extended base-pair stacking in a stretched DNA structure.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
530 Physik
Schlagwörter
DNA structure, DNA cross-linking, Raman scattering
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ANDRÉ, Alexander, Fabien FONTAINE-VIVE, Heiko M. MÖLLER, Theo FISCHER, Georg MARET, Thomas GISLER, V. Trevor FORSYTH, 2008. Force-induced structural transitions in cross-linked DNA films. In: European Biophysics Journal. 2008, 37(6), pp. 749-757. ISSN 0175-7571. Available under: doi: 10.1007/s00249-008-0257-4BibTex
@article{Andre2008-07Force-16876,
year={2008},
doi={10.1007/s00249-008-0257-4},
title={Force-induced structural transitions in cross-linked DNA films},
number={6},
volume={37},
issn={0175-7571},
journal={European Biophysics Journal},
pages={749--757},
author={André, Alexander and Fontaine-Vive, Fabien and Möller, Heiko M. and Fischer, Theo and Maret, Georg and Gisler, Thomas and Forsyth, V. Trevor}
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<dcterms:abstract xml:lang="eng">We report on the preparation and characterization of wet-spun films of sodium DNA in which intermolecular cross-links were introduced following formaldehyde treatment. Raman scattering shows that the DNA in moderately cross-linked films is mainly in the B conformation. Stretching experiments show a transition from plastic to elastomeric behavior with increasing exposure to the cross-linking agent. Elastomeric DNA films are strongly disordered. X-ray diffraction shows that stretching of moderately cross-linked films under controlled high humidity conditions results in increased molecular orientation as well as the appearance of meridional reflections at 7.4–7.8 and 8.2 Å. These reflections are not observed for any of the classical conformations associated with mixed sequence DNA, and may arise from extended base-pair stacking in a stretched DNA structure.</dcterms:abstract>
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