Publikation:

Force-induced structural transitions in cross-linked DNA films

Lade...
Vorschaubild

Dateien

maret_gisler_etal.pdf
maret_gisler_etal.pdfGröße: 999.19 KBDownloads: 299

Datum

2008

Autor:innen

André, Alexander
Fontaine-Vive, Fabien
Fischer, Theo
Forsyth, V. Trevor

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

European Biophysics Journal. 2008, 37(6), pp. 749-757. ISSN 0175-7571. Available under: doi: 10.1007/s00249-008-0257-4

Zusammenfassung

We report on the preparation and characterization of wet-spun films of sodium DNA in which intermolecular cross-links were introduced following formaldehyde treatment. Raman scattering shows that the DNA in moderately cross-linked films is mainly in the B conformation. Stretching experiments show a transition from plastic to elastomeric behavior with increasing exposure to the cross-linking agent. Elastomeric DNA films are strongly disordered. X-ray diffraction shows that stretching of moderately cross-linked films under controlled high humidity conditions results in increased molecular orientation as well as the appearance of meridional reflections at 7.4–7.8 and 8.2 Å. These reflections are not observed for any of the classical conformations associated with mixed sequence DNA, and may arise from extended base-pair stacking in a stretched DNA structure.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
530 Physik

Schlagwörter

DNA structure, DNA cross-linking, Raman scattering

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690ANDRÉ, Alexander, Fabien FONTAINE-VIVE, Heiko M. MÖLLER, Theo FISCHER, Georg MARET, Thomas GISLER, V. Trevor FORSYTH, 2008. Force-induced structural transitions in cross-linked DNA films. In: European Biophysics Journal. 2008, 37(6), pp. 749-757. ISSN 0175-7571. Available under: doi: 10.1007/s00249-008-0257-4
BibTex
@article{Andre2008-07Force-16876,
  year={2008},
  doi={10.1007/s00249-008-0257-4},
  title={Force-induced structural transitions in cross-linked DNA films},
  number={6},
  volume={37},
  issn={0175-7571},
  journal={European Biophysics Journal},
  pages={749--757},
  author={André, Alexander and Fontaine-Vive, Fabien and Möller, Heiko M. and Fischer, Theo and Maret, Georg and Gisler, Thomas and Forsyth, V. Trevor}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/16876">
    <dc:contributor>Gisler, Thomas</dc:contributor>
    <dc:creator>Forsyth, V. Trevor</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:title>Force-induced structural transitions in cross-linked DNA films</dcterms:title>
    <dc:contributor>Möller, Heiko M.</dc:contributor>
    <dc:creator>Fontaine-Vive, Fabien</dc:creator>
    <dc:contributor>André, Alexander</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Fontaine-Vive, Fabien</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16876/2/maret_gisler_etal.pdf"/>
    <dcterms:issued>2008-07</dcterms:issued>
    <dc:creator>André, Alexander</dc:creator>
    <dc:creator>Möller, Heiko M.</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-04-02T12:58:42Z</dcterms:available>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16876"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/>
    <dc:rights>terms-of-use</dc:rights>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16876/2/maret_gisler_etal.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-04-02T12:58:42Z</dc:date>
    <dcterms:abstract xml:lang="eng">We report on the preparation and characterization of wet-spun films of sodium DNA in which intermolecular cross-links were introduced following formaldehyde treatment. Raman scattering shows that the DNA in moderately cross-linked films is mainly in the B conformation. Stretching experiments show a transition from plastic to elastomeric behavior with increasing exposure to the cross-linking agent. Elastomeric DNA films are strongly disordered. X-ray diffraction shows that stretching of moderately cross-linked films under controlled high humidity conditions results in increased molecular orientation as well as the appearance of meridional reflections at 7.4–7.8 and 8.2 Å. These reflections are not observed for any of the classical conformations associated with mixed sequence DNA, and may arise from extended base-pair stacking in a stretched DNA structure.</dcterms:abstract>
    <dc:contributor>Forsyth, V. Trevor</dc:contributor>
    <dc:creator>Fischer, Theo</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: European Biophysics Journal ; 37 (2008), 6. - S. 749-757</dcterms:bibliographicCitation>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:contributor>Fischer, Theo</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Gisler, Thomas</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Maret, Georg</dc:contributor>
    <dc:creator>Maret, Georg</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen