Modification of HPV 16 E7 genes : correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2005
Autor:innen
Steinberg, Thorsten
Sehr, Peter
Osen, Wolfram
Gissmann, Lutz
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

Immunization with a codon-optimized HPV 16 E7 gene was shown to yield higher levels of E7-specific cytotoxic T cells [Liu WJ, Gao F, Zhao KN, Zhao W, Fernando GJ, Thomas R, et al. Codon modified human papillomavirus type 16 E7 DNA vaccine enhances cytotoxic T-lymphocyte induction and anti-tumour activity. Virology 2002;301:43]. Here, we sought to verify the hypothesis that there is a direct correlation between the level of protein expression and immunogenicity in mice. We generated HPV 16 E7 expression plasmids where the genes were inserted either as authentic sequence (wt) or after optimizing the codons for use in mammalian cells (opt). For enhancement of translation of the E7 gene a 5′ Kozak sequence (K) was added. Transfection experiments revealed the strength of expression in the order of E7opt + K, E7opt − K, E7wt + K and E7wt − K. After immunization of C57/B6 mice we observed an equally strong CD8 + T-cell response with the E7opt plasmids (+ or −K), followed by the E7wt + K and E7wt − K DNAs. The same difference in efficiency was obtained in tumor protection experiments. Regression of pre-existing tumors and CTL activity was observed only with the E7opt + K plasmid. From these data, we conclude that the level of protein expression correlates with the efficiency of CTL response and hence testing by transfection of cells in culture may allow a pre-selection of expression plasmids prior to DNA immunization.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
HPV 16 E7 expression clones, T-cell response
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690STEINBERG, Thorsten, Peter ÖHLSCHLÄGER, Peter SEHR, Wolfram OSEN, Lutz GISSMANN, 2005. Modification of HPV 16 E7 genes : correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice. In: Vaccine. 2005, 23(9), pp. 1149-1157. ISSN 0264-410X. Available under: doi: 10.1016/j.vaccine.2004.08.027
BibTex
@article{Steinberg2005-01-19Modif-17503,
  year={2005},
  doi={10.1016/j.vaccine.2004.08.027},
  title={Modification of HPV 16 E7 genes : correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice},
  number={9},
  volume={23},
  issn={0264-410X},
  journal={Vaccine},
  pages={1149--1157},
  author={Steinberg, Thorsten and Öhlschläger, Peter and Sehr, Peter and Osen, Wolfram and Gissmann, Lutz}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/17503">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2005-01-19</dcterms:issued>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">Immunization with a codon-optimized HPV 16 E7 gene was shown to yield higher levels of E7-specific cytotoxic T cells [Liu WJ, Gao F, Zhao KN, Zhao W, Fernando GJ, Thomas R, et al. Codon modified human papillomavirus type 16 E7 DNA vaccine enhances cytotoxic T-lymphocyte induction and anti-tumour activity. Virology 2002;301:43]. Here, we sought to verify the hypothesis that there is a direct correlation between the level of protein expression and immunogenicity in mice. We generated HPV 16 E7 expression plasmids where the genes were inserted either as authentic sequence (wt) or after optimizing the codons for use in mammalian cells (opt). For enhancement of translation of the E7 gene a 5′ Kozak sequence (K) was added. Transfection experiments revealed the strength of expression in the order of E7opt + K, E7opt − K, E7wt + K and E7wt − K. After immunization of C57/B6 mice we observed an equally strong CD8 + T-cell response with the E7opt plasmids (+ or −K), followed by the E7wt + K and E7wt − K DNAs. The same difference in efficiency was obtained in tumor protection experiments. Regression of pre-existing tumors and CTL activity was observed only with the E7opt + K plasmid. From these data, we conclude that the level of protein expression correlates with the efficiency of CTL response and hence testing by transfection of cells in culture may allow a pre-selection of expression plasmids prior to DNA immunization.</dcterms:abstract>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Gissmann, Lutz</dc:contributor>
    <dc:creator>Gissmann, Lutz</dc:creator>
    <dcterms:bibliographicCitation>Publ. in: Vaccine ; 23 (2005), 9. - S. 1149-1157</dcterms:bibliographicCitation>
    <dc:contributor>Öhlschläger, Peter</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/17503"/>
    <dc:language>eng</dc:language>
    <dc:contributor>Sehr, Peter</dc:contributor>
    <dc:creator>Steinberg, Thorsten</dc:creator>
    <dc:creator>Sehr, Peter</dc:creator>
    <dc:creator>Öhlschläger, Peter</dc:creator>
    <dc:creator>Osen, Wolfram</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:title>Modification of HPV 16 E7 genes : correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice</dcterms:title>
    <dc:contributor>Steinberg, Thorsten</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-13T08:23:43Z</dc:date>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-13T08:23:43Z</dcterms:available>
    <dc:contributor>Osen, Wolfram</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen