Profiling of bacterial adhesin : host receptor recognition by soluble immunoglobulin superfamily domains

Lade...
Vorschaubild
Dateien
Profiling_of_bacterial_adhesin.pdf
Profiling_of_bacterial_adhesin.pdfGröße: 448.41 KBDownloads: 537
Datum
2006
Autor:innen
Kuespert, Katharina
Weibel, Stephanie
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Journal of Microbiological Methods. 2006, 68(3), pp. 478-485. ISSN 0167-7012. Available under: doi: 10.1016/j.mimet.2006.10.003
Zusammenfassung

Several Gram-negative human pathogens recognize members of the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family. Pathogenic Neisseriae employ distinct isoforms of the colony opacity-associated proteins (Opa[CEA] proteins) to bind to the amino-terminal domains of CEACAMs. Here we present a novel approach to rapidly determine the CEACAM-binding properties of single bacteria. Expression of the isolated amino-terminal domains of various CEACAMs in eukaryotic cells yields soluble probes that selectively recognize Opac[CEA]-expressing bacteria in a pull-down assay format. Furthermore, by expressing soluble CEACAMs as fusions to green-fluorescent protein (CEACAM-N-GFP), CEACAM-binding bacteria can be decorated with a fluorescent label and analysed by flow cytometry allowing the specific detection of receptor binding events on the level of single bacteria. Besides its potential for rapid and quantitative analysis of pathogen-receptor interactions, this novel approach allows the detection of receptor recognition in heterogeneous bacterial populations and might represent a valuable tool for profiling the host binding capabilities of various microorganisms.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Bacterial adhesin, CEACAM, Flow cytometry, Neisseria gonorrhoeae, Neisseria meningitidis, Opa protein, Pull-down assay, Receptor recognition
Konferenz
Rezension
undefined / . - undefined, undefined
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Datensätze
Zitieren
ISO 690KUESPERT, Katharina, Stephanie WEIBEL, Christof R. HAUCK, 2006. Profiling of bacterial adhesin : host receptor recognition by soluble immunoglobulin superfamily domains. In: Journal of Microbiological Methods. 2006, 68(3), pp. 478-485. ISSN 0167-7012. Available under: doi: 10.1016/j.mimet.2006.10.003
BibTex
@article{Kuespert2006Profi-7653,
  year={2006},
  doi={10.1016/j.mimet.2006.10.003},
  title={Profiling of bacterial adhesin : host receptor recognition by soluble immunoglobulin superfamily domains},
  number={3},
  volume={68},
  issn={0167-7012},
  journal={Journal of Microbiological Methods},
  pages={478--485},
  author={Kuespert, Katharina and Weibel, Stephanie and Hauck, Christof R.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7653">
    <dc:creator>Kuespert, Katharina</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:05Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:36:05Z</dc:date>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Weibel, Stephanie</dc:contributor>
    <dc:creator>Weibel, Stephanie</dc:creator>
    <dc:contributor>Hauck, Christof R.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7653/1/Profiling_of_bacterial_adhesin.pdf"/>
    <dcterms:title>Profiling of bacterial adhesin : host receptor recognition by soluble immunoglobulin superfamily domains</dcterms:title>
    <dc:creator>Hauck, Christof R.</dc:creator>
    <dcterms:issued>2006</dcterms:issued>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7653/1/Profiling_of_bacterial_adhesin.pdf"/>
    <dc:contributor>Kuespert, Katharina</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Journal of Microbiological Methods 68 (2006), 3, pp. 478-485</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">Several Gram-negative human pathogens recognize members of the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family. Pathogenic Neisseriae employ distinct isoforms of the colony opacity-associated proteins (Opa[CEA] proteins) to bind to the amino-terminal domains of CEACAMs. Here we present a novel approach to rapidly determine the CEACAM-binding properties of single bacteria. Expression of the isolated amino-terminal domains of various CEACAMs in eukaryotic cells yields soluble probes that selectively recognize Opac[CEA]-expressing bacteria in a pull-down assay format. Furthermore, by expressing soluble CEACAMs as fusions to green-fluorescent protein (CEACAM-N-GFP), CEACAM-binding bacteria can be decorated with a fluorescent label and analysed by flow cytometry allowing the specific detection of receptor binding events on the level of single bacteria. Besides its potential for rapid and quantitative analysis of pathogen-receptor interactions, this novel approach allows the detection of receptor recognition in heterogeneous bacterial populations and might represent a valuable tool for profiling the host binding capabilities of various microorganisms.</dcterms:abstract>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7653"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen