A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant

Parameswaran, Aravind; Leitenmaier, Barbara; Yang, Mingjie; Kroneck, Peter M. H.; Welte, Wolfram; Lutz, Gabriela; Papoyan, Ashot; Kochian, Leon V.; Küpper, Hendrik

A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant

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Datum

2007

Autor:innen

Parameswaran, Aravind

Leitenmaier, Barbara

Yang, Mingjie

Kroneck, Peter M. H.

Welte, Wolfram

Lutz, Gabriela

Papoyan, Ashot

Kochian, Leon V.

Küpper, Hendrik

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Biochemical and Biophysical Research Communications. 2007, 363(1), pp. 51-56. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2007.08.105

Zusammenfassung

We report here the first purification of a P1B type ATPase, a group of transporters that occurs in bacteria, plants and animals incl. humans, from a eukaryotic organism in native state. TcHMA4 is a P1B type ATPase that is highly expressed in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens and contains a C-terminal 9-histidine repeat. After isolation from roots, we purified TcHMA4 protein via metal affinity chromatography. The purified protein exhibited Cd- and Zn-activated ATPase activity after reconstitution into lipid vesicles, showing that it was in its native state. Gels of crude root extract and of the purified protein revealed TcHMA4-specific bands of about 50 and 60 kDa, respectively, while the TcHMA4 mRNA predicts a single protein with a size of 128 kDa. This indicates the occurrence of post-translational processing; the properties of the two bands were characterised by their activity and binding properties.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

PARAMESWARAN, Aravind, Barbara LEITENMAIER, Mingjie YANG, Peter M. H. KRONECK, Wolfram WELTE, Gabriela LUTZ, Ashot PAPOYAN, Leon V. KOCHIAN, Hendrik KÜPPER, 2007. A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant. In: Biochemical and Biophysical Research Communications. 2007, 363(1), pp. 51-56. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2007.08.105

BibTex

@article{Parameswaran2007-11-09nativ-15523,
  year={2007},
  doi={10.1016/j.bbrc.2007.08.105},
  title={A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant},
  number={1},
  volume={363},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={51--56},
  author={Parameswaran, Aravind and Leitenmaier, Barbara and Yang, Mingjie and Kroneck, Peter M. H. and Welte, Wolfram and Lutz, Gabriela and Papoyan, Ashot and Kochian, Leon V. and Küpper, Hendrik}
}

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