A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant

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2007
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Parameswaran, Aravind
Yang, Mingjie
Lutz, Gabriela
Papoyan, Ashot
Kochian, Leon V.
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We report here the first purification of a P1B type ATPase, a group of transporters that occurs in bacteria, plants and animals incl. humans, from a eukaryotic organism in native state. TcHMA4 is a P1B type ATPase that is highly expressed in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens and contains a C-terminal 9-histidine repeat. After isolation from roots, we purified TcHMA4 protein via metal affinity chromatography. The purified protein exhibited Cd- and Zn-activated ATPase activity after reconstitution into lipid vesicles, showing that it was in its native state. Gels of crude root extract and of the purified protein revealed TcHMA4-specific bands of about 50 and 60 kDa, respectively, while the TcHMA4 mRNA predicts a single protein with a size of 128 kDa. This indicates the occurrence of post-translational processing; the properties of the two bands were characterised by their activity and binding properties.

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ISO 690PARAMESWARAN, Aravind, Barbara LEITENMAIER, Mingjie YANG, Peter M. H. KRONECK, Wolfram WELTE, Gabriela LUTZ, Ashot PAPOYAN, Leon V. KOCHIAN, Hendrik KÜPPER, 2007. A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant. In: Biochemical and Biophysical Research Communications. 2007, 363(1), pp. 51-56. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2007.08.105
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@article{Parameswaran2007-11-09nativ-15523,
  year={2007},
  doi={10.1016/j.bbrc.2007.08.105},
  title={A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant},
  number={1},
  volume={363},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={51--56},
  author={Parameswaran, Aravind and Leitenmaier, Barbara and Yang, Mingjie and Kroneck, Peter M. H. and Welte, Wolfram and Lutz, Gabriela and Papoyan, Ashot and Kochian, Leon V. and Küpper, Hendrik}
}
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