Apomictic and Sexual Ovules of Boechera Display Heterochronic Global Gene Expression Patterns

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2010
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Sharbel, Timothy F.
Voigt, Marie-Luise
Corral, José M.
Galla, Giulio
Kumlehn, Jochen
Klukas, Christian
Vogel, Heiko
Rotter, Björn
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The Plant Cell. 2010, 22(3), pp. 655-671. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.109.072223
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We have compared the transcriptomic profiles of microdissected live ovules at four developmental stages between a diploid sexual and diploid apomictic Boechera. We sequenced >2 million SuperSAGE tags and identified (1) heterochronic tags (n = 595) that demonstrated significantly different patterns of expression between sexual and apomictic ovules across all developmental stages, (2) stage-specific tags (n = 577) that were found in a single developmental stage and differentially expressed between the sexual and apomictic ovules, and (3) sex-specific (n = 237) and apomixis-specific (n = 1106) tags that were found in all four developmental stages but in only one reproductive mode. Most heterochronic and stage-specific tags were significantly downregulated during early apomictic ovule development, and 110 were associated with reproduction. By contrast, most late stage-specific tags were upregulated in the apomictic ovules, likely the result of increased gene copy number in apomictic (hexaploid) versus sexual (triploid) endosperm or of parthenogenesis. Finally, we show that apomixis-specific gene expression is characterized by a significant overrepresentation of transcription factor activity. We hypothesize that apomeiosis is associated with global downregulation at the megaspore mother cell stage. As the diploid apomict analyzed here is an ancient hybrid, these data are consistent with the postulated link between hybridization and asexuality and provide a hypothesis for multiple evolutionary origins of apomixis in the genus Boechera.

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ISO 690SHARBEL, Timothy F., Marie-Luise VOIGT, José M. CORRAL, Giulio GALLA, Jochen KUMLEHN, Christian KLUKAS, Falk SCHREIBER, Heiko VOGEL, Björn ROTTER, 2010. Apomictic and Sexual Ovules of Boechera Display Heterochronic Global Gene Expression Patterns. In: The Plant Cell. 2010, 22(3), pp. 655-671. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.109.072223
BibTex
@article{Sharbel2010-04-23Apomi-40223,
  year={2010},
  doi={10.1105/tpc.109.072223},
  title={Apomictic and Sexual Ovules of Boechera Display Heterochronic Global Gene Expression Patterns},
  number={3},
  volume={22},
  issn={1040-4651},
  journal={The Plant Cell},
  pages={655--671},
  author={Sharbel, Timothy F. and Voigt, Marie-Luise and Corral, José M. and Galla, Giulio and Kumlehn, Jochen and Klukas, Christian and Schreiber, Falk and Vogel, Heiko and Rotter, Björn}
}
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