Publikation:

Combined Noninvasive Imaging and Modeling Approaches Reveal Metabolic Compartmentation in the Barley Endosperm

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2012

Autor:innen

Rolletschek, Hardy
Melkus, Gerd
Grafahrend-Belau, Eva
Heinzel, Nicolas
Jakob, Peter M.
Borisjuk, Ljudmilla

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

The Plant Cell. 2012, 23(8), pp. 3041-3054. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.111.087015

Zusammenfassung

The starchy endosperm of cereals is a priori taken as a metabolically uniform tissue. By applying a noninvasive assay based on (13)C/(1)H-magnetic resonance imaging (MRI) to barley (Hordeum vulgare) grains, we uncovered metabolic compartmentation in the endosperm. (13)C-Suc feeding during grain filling showed that the primary site of Ala synthesis was the central region of the endosperm, the part of the caryopsis experiencing the highest level of hypoxia. Region-specific metabolism in the endosperm was characterized by flux balance analysis (FBA) and metabolite profiling. FBA predicts that in the central region of the endosperm, the tricarboxylic acid cycle shifts to a noncyclic mode, accompanied by elevated glycolytic flux and the accumulation of Ala. The metabolic compartmentation within the endosperm is advantageous for the grain's carbon and energy economy, with a prominent role being played by Ala aminotransferase. An investigation of caryopses with a genetically perturbed tissue pattern demonstrated that Ala accumulation is a consequence of oxygen status, rather than being either tissue specific or dependent on the supply of Suc. Hence the (13)C-Ala gradient can be used as an in vivo marker for hypoxia. The combination of MRI and metabolic modeling offers opportunities for the noninvasive analysis of metabolic compartmentation in plants.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
004 Informatik

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690ROLLETSCHEK, Hardy, Gerd MELKUS, Eva GRAFAHREND-BELAU, Johannes FUCHS, Nicolas HEINZEL, Falk SCHREIBER, Peter M. JAKOB, Ljudmilla BORISJUK, 2012. Combined Noninvasive Imaging and Modeling Approaches Reveal Metabolic Compartmentation in the Barley Endosperm. In: The Plant Cell. 2012, 23(8), pp. 3041-3054. ISSN 1040-4651. eISSN 1532-298X. Available under: doi: 10.1105/tpc.111.087015
BibTex
@article{Rolletschek2012-08-24Combi-38228,
  year={2012},
  doi={10.1105/tpc.111.087015},
  title={Combined Noninvasive Imaging and Modeling Approaches Reveal Metabolic Compartmentation in the Barley Endosperm},
  number={8},
  volume={23},
  issn={1040-4651},
  journal={The Plant Cell},
  pages={3041--3054},
  author={Rolletschek, Hardy and Melkus, Gerd and Grafahrend-Belau, Eva and Fuchs, Johannes and Heinzel, Nicolas and Schreiber, Falk and Jakob, Peter M. and Borisjuk, Ljudmilla}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/38228">
    <dc:contributor>Grafahrend-Belau, Eva</dc:contributor>
    <dc:creator>Schreiber, Falk</dc:creator>
    <dcterms:issued>2012-08-24</dcterms:issued>
    <dc:contributor>Jakob, Peter M.</dc:contributor>
    <dc:creator>Rolletschek, Hardy</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-03-30T09:37:05Z</dcterms:available>
    <dc:contributor>Melkus, Gerd</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-03-30T09:37:05Z</dc:date>
    <dc:creator>Heinzel, Nicolas</dc:creator>
    <dc:creator>Jakob, Peter M.</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38228"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Heinzel, Nicolas</dc:contributor>
    <dc:contributor>Fuchs, Johannes</dc:contributor>
    <dc:contributor>Schreiber, Falk</dc:contributor>
    <dcterms:title>Combined Noninvasive Imaging and Modeling Approaches Reveal Metabolic Compartmentation in the Barley Endosperm</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/36"/>
    <dcterms:abstract xml:lang="eng">The starchy endosperm of cereals is a priori taken as a metabolically uniform tissue. By applying a noninvasive assay based on (13)C/(1)H-magnetic resonance imaging (MRI) to barley (Hordeum vulgare) grains, we uncovered metabolic compartmentation in the endosperm. (13)C-Suc feeding during grain filling showed that the primary site of Ala synthesis was the central region of the endosperm, the part of the caryopsis experiencing the highest level of hypoxia. Region-specific metabolism in the endosperm was characterized by flux balance analysis (FBA) and metabolite profiling. FBA predicts that in the central region of the endosperm, the tricarboxylic acid cycle shifts to a noncyclic mode, accompanied by elevated glycolytic flux and the accumulation of Ala. The metabolic compartmentation within the endosperm is advantageous for the grain's carbon and energy economy, with a prominent role being played by Ala aminotransferase. An investigation of caryopses with a genetically perturbed tissue pattern demonstrated that Ala accumulation is a consequence of oxygen status, rather than being either tissue specific or dependent on the supply of Suc. Hence the (13)C-Ala gradient can be used as an in vivo marker for hypoxia. The combination of MRI and metabolic modeling offers opportunities for the noninvasive analysis of metabolic compartmentation in plants.</dcterms:abstract>
    <dc:creator>Fuchs, Johannes</dc:creator>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/36"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Borisjuk, Ljudmilla</dc:creator>
    <dc:contributor>Rolletschek, Hardy</dc:contributor>
    <dc:creator>Melkus, Gerd</dc:creator>
    <dc:contributor>Borisjuk, Ljudmilla</dc:contributor>
    <dc:creator>Grafahrend-Belau, Eva</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen