Publikation:

Dynamics of Trigger Factor Interaction with Translating Ribosomes

Lade...
Vorschaubild

Dateien

Rutkowska_JBC2008.pdf
Rutkowska_JBC2008.pdfGröße: 662.54 KBDownloads: 235

Datum

2008

Autor:innen

Rutkowska, Anna
Mayer, Matthias P.
Hoffmann, Anja
Merz, Frieder
Zachmann-Brand, Beate
Schaffitzel, Christiane
Ban, Nenad
Bukau, Bernd

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of Biological Chemistry. 2008, 283(7), pp. 4124-4132. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M708294200

Zusammenfassung

In all organisms ribosome-associated chaperones assist early steps of protein folding. To elucidate the mechanism of their action, we determined the kinetics of individual steps of the ribosome binding/release cycle of bacterial trigger factor (TF), using fluorescently labeled chaperone and ribosome-nascent chain complexes. Both the association and dissociation rates of TF-ribosome complexes are modulated by nascent chains, whereby their length, sequence, and folding status are influencing parameters. However, the effect of the folding status is modest, indicating that TF can bind small globular domains and accommodate them within its substrate binding cavity. In general, the presence of a nascent chain causes an up to 9-fold increase in the rate of TF association, which provides a kinetic explanation for the observed ability of TF to efficiently compete with other cytosolic chaperones for binding to nascent chains. Furthermore, a subset of longer nascent polypeptides promotes the stabilization of TF-ribosome complexes, which increases the half-life of these complexes from 15 to 50 s. Nascent chains thus regulate their folding environment generated by ribosome associated chaperones.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690RUTKOWSKA, Anna, Matthias P. MAYER, Anja HOFFMANN, Frieder MERZ, Beate ZACHMANN-BRAND, Christiane SCHAFFITZEL, Nenad BAN, Elke DEUERLING, Bernd BUKAU, 2008. Dynamics of Trigger Factor Interaction with Translating Ribosomes. In: Journal of Biological Chemistry. 2008, 283(7), pp. 4124-4132. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M708294200
BibTex
@article{Rutkowska2008Dynam-6697,
  year={2008},
  doi={10.1074/jbc.M708294200},
  title={Dynamics of Trigger Factor Interaction with Translating Ribosomes},
  number={7},
  volume={283},
  issn={0021-9258},
  journal={Journal of Biological Chemistry},
  pages={4124--4132},
  author={Rutkowska, Anna and Mayer, Matthias P. and Hoffmann, Anja and Merz, Frieder and Zachmann-Brand, Beate and Schaffitzel, Christiane and Ban, Nenad and Deuerling, Elke and Bukau, Bernd}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6697">
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Zachmann-Brand, Beate</dc:creator>
    <dc:contributor>Mayer, Matthias P.</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:title>Dynamics of Trigger Factor Interaction with Translating Ribosomes</dcterms:title>
    <dc:creator>Merz, Frieder</dc:creator>
    <dc:contributor>Hoffmann, Anja</dc:contributor>
    <dcterms:abstract xml:lang="eng">In all organisms ribosome-associated chaperones assist early steps of protein folding. To elucidate the mechanism of their action, we determined the kinetics of individual steps of the ribosome binding/release cycle of bacterial trigger factor (TF), using fluorescently labeled chaperone and ribosome-nascent chain complexes. Both the association and dissociation rates of TF-ribosome complexes are modulated by nascent chains, whereby their length, sequence, and folding status are influencing parameters. However, the effect of the folding status is modest, indicating that TF can bind small globular domains and accommodate them within its substrate binding cavity. In general, the presence of a nascent chain causes an up to 9-fold increase in the rate of TF association, which provides a kinetic explanation for the observed ability of TF to efficiently compete with other cytosolic chaperones for binding to nascent chains. Furthermore, a subset of longer nascent polypeptides promotes the stabilization of TF-ribosome complexes, which increases the half-life of these complexes from 15 to 50 s. Nascent chains thus regulate their folding environment generated by ribosome associated chaperones.</dcterms:abstract>
    <dc:contributor>Schaffitzel, Christiane</dc:contributor>
    <dc:creator>Bukau, Bernd</dc:creator>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Ban, Nenad</dc:contributor>
    <dc:creator>Deuerling, Elke</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:27Z</dc:date>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Hoffmann, Anja</dc:creator>
    <dc:contributor>Deuerling, Elke</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6697/1/Rutkowska_JBC2008.pdf"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Zachmann-Brand, Beate</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6697/1/Rutkowska_JBC2008.pdf"/>
    <dc:creator>Ban, Nenad</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Journal of Biological Chemistry 283 (2008), 7, pp. 4124 4132</dcterms:bibliographicCitation>
    <dc:contributor>Bukau, Bernd</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6697"/>
    <dcterms:issued>2008</dcterms:issued>
    <dc:contributor>Rutkowska, Anna</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:27Z</dcterms:available>
    <dc:creator>Mayer, Matthias P.</dc:creator>
    <dc:contributor>Merz, Frieder</dc:contributor>
    <dc:creator>Rutkowska, Anna</dc:creator>
    <dc:creator>Schaffitzel, Christiane</dc:creator>
    <dc:language>deu</dc:language>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen