Publikation: Isolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction
Lade...
Dateien
Datum
1987
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biochimica et Biophysica Acta. 1987, 924(1), pp. 104-110. ISSN 0304-4165. Available under: doi: 10.1016/0304-4165(87)90076-6
Zusammenfassung
Procedures and conditions have been established such that the unstable enzyme-bound flavin intermediate produced in the bacterial luciferase reaction can be isolated as approximately 70% of the flavin product, the remaining being the final product, FMN. The structure of the intermediate is proposed to be that of a luciferase-bound 4a,5-dihydroflavin-4a-hydroxide. The intermediate has a half-life of 33 min at 2°C and decays spontaneously to give H2O and luciferase-bound FMN with an activation enthalpy of about 120 kJ/mol. It has an absorption spectrum (λmax = 360 nm) that is consistent with the proposed structure, and a fluorescence emission (λmax = 485 nm) that matches the bioluminescence emission closely.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Bioluminescence, Oxygen activation, Flavin intermediate, Luciferase, Bacteria
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
KURFÜRST, Manfred, Peter MACHEROUX, Sandro GHISLA, J. Woodland HASTINGS, 1987. Isolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction. In: Biochimica et Biophysica Acta. 1987, 924(1), pp. 104-110. ISSN 0304-4165. Available under: doi: 10.1016/0304-4165(87)90076-6BibTex
@article{Kurfurst1987Isola-7258,
year={1987},
doi={10.1016/0304-4165(87)90076-6},
title={Isolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction},
number={1},
volume={924},
issn={0304-4165},
journal={Biochimica et Biophysica Acta},
pages={104--110},
author={Kurfürst, Manfred and Macheroux, Peter and Ghisla, Sandro and Hastings, J. Woodland}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7258">
<dc:creator>Macheroux, Peter</dc:creator>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:02Z</dc:date>
<dc:creator>Kurfürst, Manfred</dc:creator>
<dc:creator>Hastings, J. Woodland</dc:creator>
<dcterms:title>Isolation and characterization of the transient, luciferase-bound flavin-4a-hydroxide in the bacterial luciferase reaction</dcterms:title>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dc:creator>Ghisla, Sandro</dc:creator>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:02Z</dcterms:available>
<dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
<dc:contributor>Macheroux, Peter</dc:contributor>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7258/1/ghislascan154.pdf"/>
<dc:language>eng</dc:language>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:contributor>Kurfürst, Manfred</dc:contributor>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7258"/>
<dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7258/1/ghislascan154.pdf"/>
<dc:contributor>Ghisla, Sandro</dc:contributor>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dcterms:abstract xml:lang="eng">Procedures and conditions have been established such that the unstable enzyme-bound flavin intermediate produced in the bacterial luciferase reaction can be isolated as approximately 70% of the flavin product, the remaining being the final product, FMN. The structure of the intermediate is proposed to be that of a luciferase-bound 4a,5-dihydroflavin-4a-hydroxide. The intermediate has a half-life of 33 min at 2°C and decays spontaneously to give H2O and luciferase-bound FMN with an activation enthalpy of about 120 kJ/mol. It has an absorption spectrum (λmax = 360 nm) that is consistent with the proposed structure, and a fluorescence emission (λmax = 485 nm) that matches the bioluminescence emission closely.</dcterms:abstract>
<dc:contributor>Hastings, J. Woodland</dc:contributor>
<dcterms:bibliographicCitation>First publ. in: Biochimica et Biophysica Acta 924 (1987), 1, pp. 104-110</dcterms:bibliographicCitation>
<dcterms:issued>1987</dcterms:issued>
<dc:format>application/pdf</dc:format>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
