Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone
Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone
Loading...
Date
1998
Authors
Gallus, Corinna
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
Archives of Microbiology ; 169 (1998), 4. - pp. 333-338. - ISSN 0302-8933. - eISSN 1432-072X
Abstract
Anaerobic degradation of alpha-resorcylate (3,5-dihydroxybenzoate) was studied with the denitrifying strain AR-1, which was assigned to the described species Thauera aromatica. Alpha-Resorcylate degradation does not proceed via the benzoyl-CoA, the resorcinol, or the phloroglucinol pathway. Instead, a-resorcylate is converted to hydroxyhydroquinone (1,2,4-trihydroxybenzene) by dehydrogenative oxidation and decarboxylation. Nitrate, K3[Fe(CN)6], dichlorophenol indophenol, and the NAD+ analogue 3-acetylpyridine adeninedinucleotide were suitable electron acceptors for the oxidation reaction; NAD+ did not function as an electron acceptor. The oxidation reaction was strongly accelerated by the additional presence of the redox carrier phenazine methosulfate, which could also be used as sole electron acceptor. Oxidation of alpha-resorcylate with molecular oxygen in cell suspensions or in cell-free extracts of a-resorcylate- and nitrate-grown cells was also detected although this bacterium did not grow with a-resorcylate under an air atmosphere. Alpha-Resorcylate degradation to hydroxyhydroquinone proceeded in two steps. The a-resorcylate-oxidizing enzyme activity was membrane-associated and exhibited maximal activity at pH 8.0. The primary oxidation product was not hydroxyhydroquinone. Rather, formation of hydroxyhydroquinone by decarboxylation of the unknown intermediate in addition required the cytoplasmic fraction and needed lower pH values since hydroxyhydroquinone was not stable at alkaline pH.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
alpha-resorcylate (3,5-dihydroxybenzoate),hydroxyhydroquinone (1,2,4-trihydroxybenzene),aromatic compounds,anaerobic degradation
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
GALLUS, Corinna, Bernhard SCHINK, 1998. Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone. In: Archives of Microbiology. 169(4), pp. 333-338. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s002030050579BibTex
@article{Gallus1998Anaer-8449, year={1998}, doi={10.1007/s002030050579}, title={Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone}, number={4}, volume={169}, issn={0302-8933}, journal={Archives of Microbiology}, pages={333--338}, author={Gallus, Corinna and Schink, Bernhard} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8449"> <dc:creator>Gallus, Corinna</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:46Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8449/1/Anaerobic_degradation_of_alpha_resorcylate_by_Thauera_aromaticastrain_AR_1_proceeds_via_oxidation_and_decarboxylation_to_hydroxyhydroquinone.pdf"/> <dc:contributor>Gallus, Corinna</dc:contributor> <dc:creator>Schink, Bernhard</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:46Z</dcterms:available> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8449"/> <dcterms:abstract xml:lang="eng">Anaerobic degradation of alpha-resorcylate (3,5-dihydroxybenzoate) was studied with the denitrifying strain AR-1, which was assigned to the described species Thauera aromatica. Alpha-Resorcylate degradation does not proceed via the benzoyl-CoA, the resorcinol, or the phloroglucinol pathway. Instead, a-resorcylate is converted to hydroxyhydroquinone (1,2,4-trihydroxybenzene) by dehydrogenative oxidation and decarboxylation. Nitrate, K3[Fe(CN)6], dichlorophenol indophenol, and the NAD+ analogue 3-acetylpyridine adeninedinucleotide were suitable electron acceptors for the oxidation reaction; NAD+ did not function as an electron acceptor. The oxidation reaction was strongly accelerated by the additional presence of the redox carrier phenazine methosulfate, which could also be used as sole electron acceptor. Oxidation of alpha-resorcylate with molecular oxygen in cell suspensions or in cell-free extracts of a-resorcylate- and nitrate-grown cells was also detected although this bacterium did not grow with a-resorcylate under an air atmosphere. Alpha-Resorcylate degradation to hydroxyhydroquinone proceeded in two steps. The a-resorcylate-oxidizing enzyme activity was membrane-associated and exhibited maximal activity at pH 8.0. The primary oxidation product was not hydroxyhydroquinone. Rather, formation of hydroxyhydroquinone by decarboxylation of the unknown intermediate in addition required the cytoplasmic fraction and needed lower pH values since hydroxyhydroquinone was not stable at alkaline pH.</dcterms:abstract> <dc:language>eng</dc:language> <dc:format>application/pdf</dc:format> <dc:contributor>Schink, Bernhard</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:rights>terms-of-use</dc:rights> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8449/1/Anaerobic_degradation_of_alpha_resorcylate_by_Thauera_aromaticastrain_AR_1_proceeds_via_oxidation_and_decarboxylation_to_hydroxyhydroquinone.pdf"/> <dcterms:title>Anaerobic degradation of alpha-resorcylate by Thauera aromaticastrain AR-1 proceeds via oxidation and decarboxylation to hydroxyhydroquinone</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 169 (1998), pp. 333-338</dcterms:bibliographicCitation> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:issued>1998</dcterms:issued> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No