Only a Few Fungal Species Dominate Highly Diverse Mycofloras Associated with the Common Reed

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2006
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Neubert, Karin
Brinkmann, Henner
Wirsel, Stefan G. R.
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Applied and Environmental Microbiology. 2006, 72(2), pp. 1118-1128. ISSN 0099-2240. Available under: doi: 10.1128/AEM.72.2.1118-1128.2006
Zusammenfassung

Plants are naturally colonized by many fungal species that produce effects ranging from beneficial to pathogenic. However, how many of these fungi are linked with a single host plant has not been determined. Furthermore, the composition of plant-associated fungal communities has not been rigorously determined. We investigated these essential issues by employing the perennial wetland reed Phragmites australis as a model. DNA extracted from roots, rhizomes, stems, and leaves was used for amplification and cloning of internal transcribed spacer rRNA gene fragments originating from reed-associated fungi. A total of 1,991 clones from 15 clone libraries were differentiated by restriction fragment length polymorphism analyses into 345 operational taxonomical units (OTUs). Nonparametric estimators for total richness (Chao1 and ACE) and also a parametric log normal model predicted a total of about 750 OTUs if the libraries were infinite. Sixty-two percent of the OTUs sequenced were novel at a threshold of 3%. Several of these OTUs represented undocumented fungal species, which also included higher taxonomic levels. In spite of the high diversity of the OTUs, the mycofloras of vegetative organs were dominated by just a few typical fungi, which suggested that competition and niche differentiation influence the composition of plant-associated fungal communities. This suggestion was independently supported by the results of nested PCR assays specifically monitoring two OTUs over 3 years, which revealed significant preferences for host habitat and host organ.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Phragmites australis, plant-associated fungal communities, operational taxonomical units
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ISO 690NEUBERT, Karin, Kurt MENDGEN, Henner BRINKMANN, Stefan G. R. WIRSEL, 2006. Only a Few Fungal Species Dominate Highly Diverse Mycofloras Associated with the Common Reed. In: Applied and Environmental Microbiology. 2006, 72(2), pp. 1118-1128. ISSN 0099-2240. Available under: doi: 10.1128/AEM.72.2.1118-1128.2006
BibTex
@article{Neubert2006Funga-8647,
  year={2006},
  doi={10.1128/AEM.72.2.1118-1128.2006},
  title={Only a Few Fungal Species Dominate Highly Diverse Mycofloras Associated with the Common Reed},
  number={2},
  volume={72},
  issn={0099-2240},
  journal={Applied and Environmental Microbiology},
  pages={1118--1128},
  author={Neubert, Karin and Mendgen, Kurt and Brinkmann, Henner and Wirsel, Stefan G. R.}
}
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