Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells

Lade...
Vorschaubild
Dateien
Badertscher_0-318278.pdf
Badertscher_0-318278.pdfGröße: 4.95 MBDownloads: 517
Datum
2015
Autor:innen
Badertscher, Lukas
Wild, Thomas
Montellese, Christian
Alexander, Leila T.
Bammert, Lukas
Sarazova, Marie
Stebler, Michael
Csucs, Gabor
Zamboni, Nicola
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Gold
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Cell Reports. 2015, 13(12), pp. 2879-2891. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2015.11.061
Zusammenfassung

Ribosome biogenesis is a highly complex process requiring many assisting factors. Studies in yeast have yielded comprehensive knowledge of the cellular machinery involved in this process. However, many aspects of ribosome synthesis are different in higher eukaryotes, and the global set of mammalian ribosome biogenesis factors remains unexplored. We used an imaging-based, genome-wide RNAi screen to find human proteins involved in 40S ribosomal subunit biogenesis. Our analysis identified ∼300 factors, many part of essential protein modules such as the small subunit (SSU) processome, the eIF3 and chaperonin complexes, and the ubiquitin-proteasome system. We demonstrate a role for the vertebrate-specific factor RBIS in ribosome synthesis, uncover a requirement for the CRL4 E3 ubiquitin ligase in nucleolar ribosome biogenesis, and reveal that intracellular glutamine synthesis supports 40S subunit production.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
ribosome synthesis, RNAi screening, cullin E3 ubiquitin ligase, glutamine metabolism
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690BADERTSCHER, Lukas, Thomas WILD, Christian MONTELLESE, Leila T. ALEXANDER, Lukas BAMMERT, Marie SARAZOVA, Michael STEBLER, Gabor CSUCS, Thomas U. MAYER, Nicola ZAMBONI, Ivo ZEMP, Peter HORVATH, Ulrike KUTAY, 2015. Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells. In: Cell Reports. 2015, 13(12), pp. 2879-2891. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2015.11.061
BibTex
@article{Badertscher2015Genom-33396,
  year={2015},
  doi={10.1016/j.celrep.2015.11.061},
  title={Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells},
  number={12},
  volume={13},
  journal={Cell Reports},
  pages={2879--2891},
  author={Badertscher, Lukas and Wild, Thomas and Montellese, Christian and Alexander, Leila T. and Bammert, Lukas and Sarazova, Marie and Stebler, Michael and Csucs, Gabor and Mayer, Thomas U. and Zamboni, Nicola and Zemp, Ivo and Horvath, Peter and Kutay, Ulrike}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/33396">
    <dc:rights>Attribution-NonCommercial-NoDerivatives 4.0 International</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33396/3/Badertscher_0-318278.pdf"/>
    <dc:contributor>Wild, Thomas</dc:contributor>
    <dc:creator>Zemp, Ivo</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Stebler, Michael</dc:creator>
    <dc:creator>Badertscher, Lukas</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-21T14:43:55Z</dcterms:available>
    <dc:contributor>Horvath, Peter</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Sarazova, Marie</dc:contributor>
    <dc:creator>Horvath, Peter</dc:creator>
    <dc:contributor>Badertscher, Lukas</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33396"/>
    <dc:creator>Bammert, Lukas</dc:creator>
    <dc:creator>Zamboni, Nicola</dc:creator>
    <dc:creator>Mayer, Thomas U.</dc:creator>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Bammert, Lukas</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/4.0/"/>
    <dc:creator>Alexander, Leila T.</dc:creator>
    <dc:contributor>Csucs, Gabor</dc:contributor>
    <dcterms:title>Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells</dcterms:title>
    <dc:contributor>Zamboni, Nicola</dc:contributor>
    <dc:contributor>Zemp, Ivo</dc:contributor>
    <dc:contributor>Kutay, Ulrike</dc:contributor>
    <dcterms:issued>2015</dcterms:issued>
    <dc:contributor>Mayer, Thomas U.</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Montellese, Christian</dc:creator>
    <dc:creator>Kutay, Ulrike</dc:creator>
    <dc:creator>Wild, Thomas</dc:creator>
    <dc:contributor>Stebler, Michael</dc:contributor>
    <dcterms:abstract xml:lang="eng">Ribosome biogenesis is a highly complex process requiring many assisting factors. Studies in yeast have yielded comprehensive knowledge of the cellular machinery involved in this process. However, many aspects of ribosome synthesis are different in higher eukaryotes, and the global set of mammalian ribosome biogenesis factors remains unexplored. We used an imaging-based, genome-wide RNAi screen to find human proteins involved in 40S ribosomal subunit biogenesis. Our analysis identified ∼300 factors, many part of essential protein modules such as the small subunit (SSU) processome, the eIF3 and chaperonin complexes, and the ubiquitin-proteasome system. We demonstrate a role for the vertebrate-specific factor RBIS in ribosome synthesis, uncover a requirement for the CRL4 E3 ubiquitin ligase in nucleolar ribosome biogenesis, and reveal that intracellular glutamine synthesis supports 40S subunit production.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-21T14:43:55Z</dc:date>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33396/3/Badertscher_0-318278.pdf"/>
    <dc:creator>Csucs, Gabor</dc:creator>
    <dc:creator>Sarazova, Marie</dc:creator>
    <dc:contributor>Alexander, Leila T.</dc:contributor>
    <dc:contributor>Montellese, Christian</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen